Evidence map›Paper›PMID 31009269›Full record

ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2019

Decorin Expression in Human Vulva Carcinoma: Oncosuppressive Effect of Decorin cDNA Transduction on Carcinoma Cells.

Marie C Nyman, Anne B Jokilammi, Pia C Boström, Samu H Kurki, Annele O Sainio, Seija E Grenman, Katri J Orte, Sakari H Hietanen, Klaus Elenius, Hannu T Järveläinen

Open access · bronzeAbstract read
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In one paragraph

Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Marie C NymanMedical Biochemistry and Genetics, Institute of Biomedicine, University of Turku, Turku, Finland.
Anne B JokilammiMedical Biochemistry and Genetics, Institute of Biomedicine, University of Turku, Turku, Finland.
Pia C BoströmDepartment of Pathology, University of Turku, Turku, Finland.
Samu H KurkiAuria Biobank, University of Turku, Turku, Finland.
Annele O SainioMedical Biochemistry and Genetics, Institute of Biomedicine, University of Turku, Turku, Finland.
Seija E GrenmanDepartment of Obstetrics and Gynecology, Turku University Hospital, Turku, Finland.
Katri J OrteDepartment of Pathology, University of Turku, Turku, Finland.
Sakari H HietanenDepartment of Obstetrics and Gynecology, Turku University Hospital, Turku, Finland.
Klaus EleniusMedical Biochemistry and Genetics, Institute of Biomedicine, University of Turku, Turku, Finland.
Hannu T JärveläinenMedical Biochemistry and Genetics, Institute of Biomedicine, University of Turku, Turku, Finland.
University of Turku · FITurku University Hospital · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The extracellular matrix proteoglycan decorin is well-known for its oncosuppressive activity. Here, decorin expression was examined in human vulva carcinoma tissue samples and in primary and commercial cell lines representing this malignant disease. Furthermore, the effect of adenovirus-mediated decorin cDNA (Ad-DCN) transduction on the viability, proliferation, and the expression and activity of the epidermal growth factor receptor (ErbB/HER) family members of the cell lines were investigated. Using in situ hybridization and immunohistochemistry for decorin, it was demonstrated that malignant cells in human vulva carcinoma tissues lack decorin expression. This result was true independently on tumor stage, grade or human papillomavirus status. RT-qPCR analyses showed that the human vulva carcinoma cell lines used in this study were also negative for decorin expression. Transduction of the cell lines with Ad-DCN caused a marked reduction in cell viability, while the proliferation of the cells was not affected. Experiments examining potential mechanisms behind the oncosuppressive effect of Ad-DCN transduction revealed that ErbB2/HER2 expression and activity in carcinoma cells were markedly downregulated. In conclusion, the results of this study showed that human vulva carcinoma cells lack decorin expression, and that Ad-DCN transduction of these cells induces oncosuppressive activity in part via downregulation of ErbB2/HER2.

Indexed as

Gene Expression Regulation, NeoplasticTransduction, GeneticAdultAgedAged, 80 and overCell Line, TumorDecorinDNA, ComplementaryErbB ReceptorsFemaleGenital Neoplasms, FemaleHumansMiddle AgedProto-Oncogene Proteins c-aktRNA, MessengerSequestosome-1 ProteinDecorinDNA, ComplementaryEGFR protein, humanErbB ReceptorsProto-Oncogene Proteins c-aktRNA, MessengerSequestosome-1 ProteinSQSTM1 protein, humanDecorin cDNAErbB2/HER2oncosuppressivetransductionvulva carcinoma

Identifiers

PMID31009269
PMCPMC6598130
OpenAlexW2942093576

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.