ArticleSynthetic biology (Oxford, England)2019
CRISPR-interference-based modulation of mobile genetic elements in bacteria.
Article in Synthetic biology (Oxford, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Probing the limits of genetic recoding using multi-omics-guided evolution.Nature communications · 2026Article
- Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology.Nature communications · 2024Article
- Synthetic genomes unveil the effects of synonymous recoding.bioRxiv : the preprint server for biology · 2024Article
- Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology.bioRxiv : the preprint server for biology · 2024Article
- Diverse mechanisms of bioproduction heterogeneity in fermentation and their control strategies.Journal of industrial microbiology & biotechnology · 2023Review
- Frequency, composition and mobility of Escherichia coli-derived transposable elements in holdings of plasmid repositories.Microbial biotechnology · 2022Article
- Review
- Design patterns for engineering genetic stability.Current opinion in biomedical engineering · 2021Article
- Towards an engineering theory of evolution.Nature communications · 2021Review
- Genome Plasticity by Insertion Sequences Learned From a Case of Radiation-Resistant BacteriumBioinformatics and biology insights · 2021Article
- Article
- The future of self-selecting and stable fermentations.Journal of industrial microbiology & biotechnology · 2020Review
- Overcoming genetic heterogeneity in industrial fermentations.Nature biotechnology · 2019Review
- A single plasmid based CRISPR interference in Synechocystis 6803 - A proof of concept.PloS one · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Spontaneous mutagenesis of synthetic genetic constructs by mobile genetic elements frequently results in the rapid loss of engineered functions. Previous efforts to minimize such mutations required the exceedingly time-consuming manipulation of bacterial chromosomes and the complete removal of insertional sequences (ISes). To this aim, we developed a single plasmid-based system (pCRIS) that applies CRISPR-interference to inhibit the transposition of bacterial ISes. pCRIS expresses multiple guide RNAs to direct inactivated Cas9 (dCas9) to simultaneously silence IS
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.