Evidence map›Paper›PMID 31007314›Full record

ArticleIEEE MTT-S International Microwave Symposium digest. IEEE MTT-S International Microwave Symposium2018

Scanning Microwave Microscopy of Vital Mitochondria in Respiration Buffer.

Jinfeng Li, Zahra Nemati, Kamel Haddadi, Douglas C Wallace, Peter J Burke

Open access · greenAbstract read
In one paragraph

Article in IEEE MTT-S International Microwave Symposium digest. IEEE MTT-S International Microwave Symposium, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 21 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Jinfeng LiDepartment of Physics and Astronomy, University of California, Irvine, Irvine, CA 92697, USA.
Zahra NematiDepartment of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, CA 92697, USA.
Kamel HaddadiInstitute of Electronics, Microelectronics and Nanotechnology, University of Lille, Villeneuve d'Ascq, 59652 France.
Douglas C WallaceCenter for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Peter J BurkeIntegrated Nanosystems Research Facility, University of California, Irvine, Irvine, CA 92697, USA.
University of California, Irvine · USUniversité de Lille · FRUniversity of Pennsylvania · US

Funding

High-throughput on-chip platform for interrogation of mitochondrial membrane potential and apoptosisR33CA182384 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI BURKE, PETER J. · 2015 to 2017
$1.2M
NCI NIH HHS R33 CA182384
6 · The paper itself

Abstract

We demonstrate imaging using scanning microwave microscopy (SMM) of vital mitochondria in respiration buffer. The mitochondria are isolated from cultured HeLa cells and tethered to a solid graphene support. The mitochondria are kept vital (alive) using a respiration buffer, which provides nutrients to sustain the Krebs cycle. We verify that the mitochondria are "alive" by measuring the membrane potential using a voltage sensitive fluorescent dye (TMRE). The organelles are measured capacitively at 7 GHz. Several technical advances are demonstrated which enable this work: 1) The SMM operates in an electrophysiologically relevant liquid (hence conducting) environment; 2) The SMM operates in tapping mode, averaging the microwave reflection measurement over many tapping periods; 3) A tuned reflectometer enables increased sensitivity; 4) Variable frequencies up to 18 GHz are used; 5) In contrast with traditional matching/resonant methods that exhibit high quality factor that fail in the presence of liquids, interferometric/tuned reflectometer gives the possibility to adjust the quality factor or sensitivity even in the presence of the liquid.

Identifiers

PMID31007314
PMCPMC6469850
OpenAlexW2962919907

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.