ArticleJournal of thrombosis and haemostasis : JTH2019
Temperature-dependent irreversible conformational change of recombinant ADAMTS13 upon metal ion chelation.
Article in Journal of thrombosis and haemostasis : JTH, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- Optimization of plasma-based BioID identifies plasminogen as a ligand of ADAMTS13.Scientific reports · 2024Article
- Analysis of the sensitization activity ofFrontiers in nutrition · 2024Article
- ADAMTS13 Activity: Screening Test Protocol.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Temperature-dependent irreversible conformational change of recombinant ADAMTS13 upon metal ion chelation.Journal of thrombosis and haemostasis : JTH · 2019Article
- Bethesda Assay for Detecting Inhibitory Anti-ADAMTS13 Antibodies in Immune-Mediated Thrombotic Thrombocytopenic Purpura.TH open : companion journal to thrombosis and haemostasis · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe catalytic domain of ADAMTS13 possesses one Zn
objectivesTo address the stability of ADAMTS13 in citrated human plasma.
methodsADAMTS13 activity was measured using the FRETS-VWF73 fluorogenic assay. The molar ratio of metals bound to ADAMTS13 was determined by size exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS). Higher-order structural changes were analyzed using Fourier-transformed infrared spectroscopy and dynamic light scattering.
resultsADAMTS13 was stable at room temperature for up to 24 hours irrespective of the presence of citrate (0.38%). However, at 37°C, citrate caused a time-dependent activity decrease. No ADAMTS13 activity decrease was seen in heparinized plasma, but the addition of citrate again caused ADAMTS13 instability at 37°C. Scavenging of citrate by the addition of Ca
conclusionsZn
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Registered trials
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