ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019
Rapid detection of Salmonella contamination in seafoods using multiplex PCR.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Loop-Mediated Isothermal Amplification (LAMP) as a Rapid Molecular Tool for Salmonella Detection in Food.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A Simple, Rapid and Reliable Protocol for Extraction of High Quality Bacterial Genomic DNA Directly from Potato Tubers for Efficient PCR-Based Surveillance and Molecular Characterization ofMethods and protocols · 2026Article
- Using Risk-Benefit Analysis and the Analytical Hierarchy Process to Decide on the Implementation of Rapid Salmonella Detection Methods in Large Poultry Industries.Risk analysis : an official publication of the Society for Risk Analysis · 2026Article
- Phenotypic and molecular characterization of Salmonella enterica isolated from retail beef in Peshawar, Pakistan.PloS one · 2026Article
- Analysis of the current status of genetic diversity in Liaoning Huoyan goose based on microsatellite markers.Journal, genetic engineering & biotechnology · 2025Article
- The Unique Capability of Endolysin to Tackle Antibiotic Resistance: Cracking the Barrier.Journal of xenobiotics · 2025Review
- Exploring vaginal microbiome: from traditional methods to metagenomic next-generation sequencing-a systematic review.Frontiers in microbiology · 2025Review
- Toward same-day detection ofFrontiers in microbiology · 2025Article
- Establishment and application of a dual RPA-LFD rapid detection method for Salmonella Pullorum and Salmonella Enteritidis.PloS one · 2025Article
- Clinical detection of four emerging canine diarrhea-associated viruses and evolutionary analysis of canine kobuvirus.Archives of virology · 2024Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- A dual RPA-LFD assay for the simultaneous detection ofFrontiers in bioengineering and biotechnology · 2024Article
- Salmonella detection with LAMP and qPCR and identification of serovars of interest by multiplex qPCR in poultry carcasses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- A Comprehensive Review for the Surveillance of Human Pathogenic Microorganisms in Shellfish.Microorganisms · 2023Review
- Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi.Journal of clinical microbiology · 2023Article
- Capture and identification of bacteria from fish muscle based on immunomagnetic beads and MALDI-TOF MS.Food chemistry: X · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective monitoring of Salmonella contamination in seafood processing to conform the requirements of HACCP is a great challenge today. Such challenges can be effectively addressed, if the conventional detection methods are replaced with DNA-based molecular methods. Accordingly, it was aimed to develop a robust PCR protocol for specific detection of Salmonella spp. Out of the different primers screened, one pair of primers developed in this study targeting invA gene demonstrated 100% inclusivity for a wide range of Salmonella serotypes and 100% exclusivity for wide range of non-target species. The in silico analysis of the nucleotide sequence obtained from the PCR product suggests its potential as a hybridization probe for genus specific detection of Salmonella spp. contamination. The PCR protocol was sensitive enough to detect 15 cells per reaction using crude DNA prepared within a short time directly from artificially contaminated shrimp tissue. The study demonstrated that the result of PCR reaction can come out on the same day of sample arrival. Incorporation of this pair of primers in a multiplex PCR designed for simultaneous detection of four common seafood-borne human pathogens yielded 147 bp, 302 bp, 403 bp, and 450 bp distinct DNA bands specifically targeting E. coli, toxigenic Vibrio cholerae, Salmonella spp., and V. parahaemolyticus, respectively in a single PCR tube. The PCR methods developed in this study has the potential to be used in the seafood processing plants for effective monitoring of CCPs required for implementation of HACCP-based quality assurance system.
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