ArticlePlant molecular biology2019
Different knockout genotypes of OsIAA23 in rice using CRISPR/Cas9 generating different phenotypes.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Gene editing in cancer therapy: overcoming drug resistance and enhancing precision medicine.Cancer gene therapy · 2025Review
- Isolation ofBiology · 2025Article
- OsIAA23 Promotes Heading by Directly Downregulating Ghd7 in rice.Rice (New York, N.Y.) · 2024Article
- Escaping from CRISPR-Cas-mediated knockout: the facts, mechanisms, and applications.Cellular & molecular biology letters · 2024Review
- Auxins and grass shoot architecture: how the most important hormone makes the most important plants.Journal of experimental botany · 2023Review
- A New Function ofPlants (Basel, Switzerland) · 2023Article
- The Germin-like protein gene OsGER4 is involved in heat stress response in rice root development.Functional & integrative genomics · 2023Article
- Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants.ACS agricultural science & technology · 2022Review
- In rice splice variants that restore the reading frame after frameshifting indel introduction are common, often induced by the indels and sometimes lead to organism-level rescue.PLoS genetics · 2022Article
- SAMBA controls cell division rate during maize development.Plant physiology · 2022Article
- The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice.Rice (New York, N.Y.) · 2021Article
- Fine mapping of the BnUC2 locus related to leaf up-curling and plant semi-dwarfing in Brassica napus.BMC genomics · 2020Article
- Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley.Plants (Basel, Switzerland) · 2020Article
- Beyond Seek and Destroy: how to Generate Allelic Series Using Genome Editing Tools.Rice (New York, N.Y.) · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe have isolated several Osiaa23 rice mutants with different knockout genotypes, resulting in different phenotypes, which suggested that different genetic backgrounds or mutation types influence gene function. The Auxin/Indole-3-Acetic Acid (Aux/IAA) gene family performs critical roles in auxin signal transduction in plants. In rice, the gene OsIAA23 (Os06t0597000) is known to affect development of roots and shoots, but previous knockouts in OsIAA23 have been sterile and difficult for research continuously. Here, we isolate new Osiaa23 mutants using the CRISPR/Cas9 system in japonica (Wuyunjing24) and indica (Kasalath) rice, with extensive genome re-sequencing to confirm the absence of off-target effects. In Kasalath, mutants with a 13-amino acid deletion showed profoundly greater dwarfing, lateral root developmental disorder, and fertility deficiency, relative to mutants with a single amino acid deletion, demonstrating that those 13 amino acids in Kasalath are essential to gene function. In Wuyunjing24, we predicted that mutants with a single base-pair frameshift insertion would experience premature termination and strong phenotypic defects, but instead these lines exhibited negligible phenotypic difference and normal fertility. Through RNA-seq, we show here that new mosaic transcripts of OsIAA23 were produced de novo, which circumvented the premature termination and thereby preserved the wild-type phenotype. This finding is a notable demonstration in plants that mutants can mask loss of function CRISPR/Cas9 editing of the target gene through de novo changes in alternative splicing.
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