ArticleSensors (Basel, Switzerland)2019
A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein.
Article in Sensors (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Uncovering Analytical Patterns for Hazardous Components in Agricultural Production Systems.Foods (Basel, Switzerland) · 2025Review
- Rapid Detection of CdMolecules (Basel, Switzerland) · 2023Article
- Detection of CdToxics · 2022Article
- Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.Molecules (Basel, Switzerland) · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
- Xenobiotics-Division and Methods of Detection: A Review.Journal of xenobiotics · 2021Review
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.World journal of microbiology & biotechnology · 2021Review
- Spectroscopic Analysis of Fe Ion-Induced Fluorescence Quenching of the Green Fluorescent Protein ZsGreen.Journal of fluorescence · 2021Article
- Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance.Sensors (Basel, Switzerland) · 2021Review
- Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System.Frontiers in microbiology · 2021Article
- Construction of a mApple-D6A3-mediated biosensor for detection of heavy metal ions.AMB Express · 2020Article
- ZnAl nano layered double hydroxides for dual functional CRISPR/Cas9 delivery and enhanced green fluorescence protein biosensor.Scientific reports · 2020Article
- Spectroscopic and Structural Analysis of CuBiosensors · 2020Article
- Preparation of "Ion-Imprinting" Difunctional Magnetic Fluorescent Nanohybrid and Its Application to Detect Cadmium Ions.Sensors (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors.
Funding
Abstract
Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. Thus, we engineered new biosensors by inserting metal-binding loops (MBLs) between β-strands 9 and 10 of the eGFP, which then undergoes conformational changes upon interaction between the MBLs and targets, thereby emitting fluorescence. The two designed MLBs based on our previous study were employed as linkers between two fragments of eGFP. As a result, an
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Registered trials
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