ArticlePloS one2019
Application of BisANS fluorescent dye for developing a novel protein assay.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
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- Fluorescent molecular rotors as versatile in situ sensors for protein quantitation.Scientific reports · 2023Article
- Quantitative Proteomics Using Isobaric Labeling: A Practical Guide.Genomics, proteomics & bioinformatics · 2021Review
- Proteomics for Low Cell Numbers: How to Optimize the Sample Preparation Workflow for Mass Spectrometry Analysis.Journal of proteome research · 2021Article
- Arginase/nitric oxide modifications using live non-pathogenicJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Neurodegeneration-related beta-amyloid as autocatabolism-attenuator in aIBRO reports · 2020Article
- Modulating Insulin Fibrillation Using Engineered B-Chains with Mutated C-Termini.Biophysical journal · 2019Article
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Authors and funding
7 authors.
Funding
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Abstract
In many biology- and chemistry-related research fields and experiments the quantification of the peptide and/or protein concentration in samples are essential. Every research environment has unique requirements, e.g. metal ions, incubation times, photostability, pH, protease inhibitors, chelators, detergents, etc. A new protein assay may be adequate in different experiments beyond or instead of the well-known standard protocols (e.g. Qubit, Bradford or bicinchoninic acid) in related conceptions. Based on our previous studies, we developed a novel protein assay applying the 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt (BisANS) fluorescent dye. This molecule has several advantageous properties related to protein detection: good solubility in water, high photostability at adequate pH, quick interaction kinetics (within seconds) with proteins and no exclusionary sensitivity to the chelator, detergent and inhibitor ingredients. The protocol described in this work is highly sensitive in a large spectrum to detect protein (100-fold diluted samples) concentrations (from 0.28 up to more than 100 μg/mL). The BisANS protein assay is valid and applicable for quantification of the amount of protein in different biological and/or chemical samples.
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Registered trials
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