ArticlePloS one2019
Rapid high throughput SYBR green assay for identifying the malaria vectors Anopheles arabiensis, Anopheles coluzzii and Anopheles gambiae s.s. Giles.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Characteristics of malaria vector populations and transmission before a randomised controlled trial assessing the efficacy of next-generation insecticide-treated nets in Côte d'Ivoire.Parasites & vectors · 2025Trial
- Trial
- Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in Ghana.Molecular ecology resources · 2026Article
- Genetic Surveillance Reveals Differential Evolutionary Dynamic of Anopheles gambiae Under Contrasting Insecticidal Tools Used in Malaria Control.Molecular ecology · 2026Article
- Toxicity of ivermectin on multiple insecticide-resistant populations of Anopheles gambiae sensu lato, Aedes aegypti, and Culex mosquitoes.Parasites & vectors · 2026Article
- Herring-Based Diets Provide Robust Support forInsects · 2026Article
- Article
- Insecticide resistance and species diversity in Anopheles gambiae s.l. in Côte d'Ivoire.PloS one · 2025Article
- Copy number variants underlie major selective sweeps in insecticide resistance genes in Anopheles arabiensis.PLoS biology · 2024Article
- First detection of kdr L1014F allele in Anopheles ziemanni and Anopheles pharoensis in Cameroon and distribution of the allele in members of the Anopheles gambiae complex.Parasites & vectors · 2024Article
- Copy number variants underlie the major selective sweeps in insecticide resistance genes inbioRxiv : the preprint server for biology · 2024Article
- Article
- Susceptibility of Anopheles gambiae to Natural Plasmodium falciparum Infection: A Comparison between the Well-Established Anopheles gambiae s.s Line and a Newly Established Ugandan Anopheles gambiae s.s. Line.The American journal of tropical medicine and hygiene · 2024Article
- Pyrethroid resistance and gene expression profile of a new resistantWellcome open research · 2024Article
- Evaluation of diffuse reflectance spectroscopy for predicting age, species, and cuticular resistance of Anopheles gambiae s.l under laboratory conditions.Scientific reports · 2023Article
- Genome-wide association studies reveal novel loci associated with pyrethroid and organophosphate resistance in Anopheles gambiae and Anopheles coluzzii.Nature communications · 2023Article
- RNA-Seq-Pop: Exploiting the sequence in RNA sequencing-A Snakemake workflow reveals patterns of insecticide resistance in the malaria vector Anopheles gambiae.Molecular ecology resources · 2023Article
- Genome-wide association studies reveal novel loci associated with pyrethroid and organophosphate resistance inbioRxiv : the preprint server for biology · 2023Article
- Widespread occurrence of copy number variants and fixation of pyrethroid target site resistance inCurrent research in parasitology & vector-borne diseases · 2023Article
- Effects of Elevated Temperatures on the Growth and Development of Adult Anopheles gambiae (s.l.) (Diptera: Culicidae) Mosquitoes.Journal of medical entomology · 2022Article
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12 authors.
Funding
Abstract
The Anopheles gambiae sensu lato species complex consists of a number of cryptic species with different habitats and behaviours. These morphologically indistinct species are identified by chromosome banding. Several molecular diagnostic techniques for distinguishing between An. coluzzii and An. gambiae are still under improvement. Although, the current SINE method for identification between An. coluzzii and An. gambiae works reliably, this study describes a refinement of the SINE method to increase sensitivity for identification of An. coluzzii, An. gambiae and An. arabiensis based on amplicon dissociation curve characteristics. Field-collected samples, laboratory-reared colonies and crossed specimens of the two species were used for the design of the protocol. An. gambiae, An. coluzzii, and hybrids of the two species were sampled from Ghana and An. arabiensis from Kenya. Samples were first characterised using conventional SINE PCR method, and further assayed using SYBR green, an intercalating fluorescent dye. The three species and hybrids were clearly differentiated using the melting temperature of the dissociation curves, with derivative peaks at 72°C for An. arabiensis, 75°C for An. gambiae and 86°C for An. coluzzii. The hybrids (An. gambiae / An. coluzzii) showed both peaks. This work is the first to describe a SYBR green real time PCR method for the characterization of An. arabiensis, An. gambiae and An. coluzzii and was purposely designed for basic melt-curve analysis (rather than high-resolution melt-curve) to allow it to be used on a wide range of real-time PCR machines.
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