ArticleACS sensors2019
Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe.
Article in ACS sensors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Recent advances in chemiluminescence probes for Tumor microenvironment with applications in cancer diagnosis and therapy.Pharmaceutical science advances · 2026Review
- Targeting Tumor-Associated Hypoxia with Bioreductively Activatable Prodrug Conjugates Derived from Dihydronaphthalene, Benzosuberene, and Indole-based Inhibitors of Tubulin Polymerization.RSC medicinal chemistry · 2025Article
- A silicon rhodamine 1,2-dioxetane chemiluminophore forOrganic & biomolecular chemistry · 2025Article
- Enzymatic Activity Profiling Using an Ultrasensitive Array of Chemiluminescent Probes for Bacterial Classification and Characterization.Journal of the American Chemical Society · 2024Article
- The Molecular Basis of Organic Chemiluminescence.Biosensors · 2023Review
- In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Oxygen-Sensing Chemiluminescent Iridium(III) 1,2-Dioxetanes: Unusual Coordination and Activity.Analysis & sensing · 2023Article
- Kinetics-Based Quantification of Peroxynitrite Using the Oxidative Decarbonylation of Isatin.Analytical chemistry · 2022Article
- Chemiluminescence in Combination with Organic Photosensitizers: Beyond the Light Penetration Depth Limit of Photodynamic Therapy.International journal of molecular sciences · 2022Review
- Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity.International journal of molecular sciences · 2022Article
- Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives.Journal of pharmaceutical analysis · 2022Article
- Chemiluminescent spiroadamantane-1,2-dioxetanes: Recent advances in molecular imaging and biomarker detection.Current opinion in chemical biology · 2022Review
- Modular Access to Diverse Chemiluminescent Dioxetane-Luminophores through Convergent Synthesis.Angewandte Chemie (International ed. in English) · 2022Article
- Dark Dynamic Therapy: Photosensitization without Light Excitation Using Chemiluminescence Resonance Energy Transfer in a Dioxetane-Erythrosin B Conjugate.ACS chemical biology · 2022Article
- Chemiluminescent 1,2-Dioxetane Iridium Complexes for Near-Infrared Oxygen Sensing.Angewandte Chemie (International ed. in English) · 2022Article
- A highly selective and sensitive chemiluminescent probe for leucine aminopeptidase detectionChemical science · 2022Article
- Chemiluminescence Measurement of Reactive Sulfur and Nitrogen Species.Antioxidants & redox signaling · 2022Review
- Seeking Illumination: The Path to Chemiluminescent 1,2-Dioxetanes for Quantitative Measurements andAccounts of chemical research · 2021Article
- Design strategy of optical probes for tumor hypoxia imaging.Science China. Life sciences · 2020Review
- Development of a Split Esterase for Protein-Protein Interaction-Dependent Small-Molecule Activation.ACS central science · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Oxygenation and tissue hypoxia play critical roles in mammalian biology and contribute to aggressive phenotypes in cancerous tumors, driving research to develop accurate and easy-to-implement methods for monitoring hypoxia in living cells and animal models. This study reports the chemiluminescent probe HyCL-4-AM, which contains a nitroaromatic sensing moiety and, importantly, an acetoxymethyl (AM) ester that dramatically improves operation in cells and animals. HyCL-4-AM provides a selective 60 000-fold increase in luminescence emission in the presence of rat liver microsomes (RLM). For cellular operation, the chemiluminescence response kinetics is sharply dependent on oxygen levels, enabling highly significant and reproducible measurement of hypoxia in living cells. Whole animal imaging experiments in muscle tissue and tumor xenografts show that HyCL-4-AM can differentiate between well oxygenated muscle tissue and hypoxic tumors, demonstrating potential for monitoring tumor reoxygenation via hyperoxic treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.