Evidence map›Paper›PMID 31001872›Full record

ArticlePlant biotechnology journal2019

A gamma-thionin protein from apple, MdD1, is required for defence against S-RNase-induced inhibition of pollen tube prior to self/non-self recognition.

Zhaoyu Gu, Wei Li, James Doughty, Dong Meng, Qing Yang, Hui Yuan, Yang Li, Qiuju Chen, Jie Yu, Chun Sheng Liu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. The Core Jasmonic Acid-Signalling ModuleCurrent issues in molecular biology · 2022
    Article
  9. Article
  10. Article
  11. Review
  12. In-depth Understanding ofInternational journal of molecular sciences · 2020
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Zhaoyu GuLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Wei LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
James DoughtyDepartment of Biology and Biochemistry, University of Bath, Bath, UK.
Dong MengLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Qing YangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Hui YuanLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Yang LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Qiuju ChenLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Jie YuLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Chun Sheng LiuLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.
Tianzhong LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, China.ORCID 0000-0003-4076-231X
China Agricultural University · CNUniversity of Bath · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Apple exhibits S-RNase-mediated self-incompatibility. Although the cytotoxic effect of S-RNase inside the self-pollen tube has been studied extensively, the underlying defence mechanism in pollen tube in Rosaceae remains unclear. On exposure to stylar S-RNase, plant defence responses are activated in the pollen tube; however, how these are regulated is currently poorly understood. Here, we show that entry of both self and non-self S-RNase into pollen tubes of apple (Malus domestica) stimulates jasmonic acid (JA) production, in turn inducing the accumulation of MdMYC2 transcripts, a transcription factor in the JA signalling pathway widely considered to be involved in plant defence processes. MdMYC2 acts as a positive regulator in the pollen tube activating expression of MdD1, a gene encoding a defence protein. Importantly, MdD1 was shown to bind to the RNase activity sites of S-RNase leading to inhibition of enzymatic activity. This work provides intriguing insights into an ancient defence mechanism present in apple pollen tubes where MdD1 likely acts as a primary line of defence to inhibit S-RNase cytotoxicity prior to self/non-self recognition.

Indexed as

RibonucleasesSelf-Incompatibility in Flowering PlantsMalusPlant ProteinsPollen TubeThioninsPlant ProteinsRibonucleasesThioninsMalus domesticaMdD1plant defencepollen tube growthS-RNase

Identifiers

PMID31001872
PMCPMC6790362
OpenAlexW2940340502

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.