ArticleProceedings of the National Academy of Sciences of the United States of America2019
RNAi expression tuning, microfluidic screening, and genome recombineering for improved protein production in
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Multi-Omics Analysis Reveals Impacts of LincRNA Deletion on Yeast Protein Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Mining yeast diversity unveils novel targets for improved heterologous laccase production in Saccharomyces cerevisiae.Microbial cell factories · 2025Article
- Establishment and application of an RNAi system inFrontiers in bioengineering and biotechnology · 2025Article
- Genome-Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- EngineeringEngineering microbiology · 2024Review
- Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae.Microbial cell factories · 2024Review
- Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets.ACS synthetic biology · 2024Article
- Large scale microfluidic CRISPR screening for increased amylase secretion in yeast.Lab on a chip · 2023Article
- Comprehensive Analysis of Signal Peptides in Saccharomyces cerevisiae Reveals Features for Efficient Secretion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- Eisosome disruption by noncoding RNA deletion increases protein secretion in yeast.PNAS nexus · 2022Article
- Rapid Gene Target Tracking for Enhancing β-Carotene Production Using Flow Cytometry-Based High-Throughput Screening in Yarrowia lipolytica.Applied and environmental microbiology · 2022Article
- CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae.FEMS yeast research · 2022Article
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii.Communications biology · 2022Article
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- Transportome-wide engineering of Saccharomyces cerevisiae.Metabolic engineering · 2021Article
- Article
- Advances in RNAi-Assisted Strain Engineering inFrontiers in bioengineering and biotechnology · 2020Review
- CRISPR/Cas9 Systems for the Development ofFrontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular machinery that supports protein synthesis and secretion lies at the foundation of cell factory-centered protein production. Due to the complexity of such cellular machinery, the challenge in generating a superior cell factory is to fully exploit the production potential by finding beneficial targets for optimized strains, which ideally could be used for improved secretion of other proteins. We focused on an approach in the yeast
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.