ArticlePloS one2019
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy.G3 (Bethesda, Md.) · 2026Article
- Integrated Functional Characterization of Hemileia vastatrix Effector Candidates Reveals Coordinated Immune Suppression, Sequential Deployment and Compartment-Specific Targeting.Molecular plant pathology · 2026Article
- Advancing fungal phylogenetics: integrating modern sequencing, dark taxa discovery, and machine learning.Archives of microbiology · 2025Review
- Developing a crop- wild-reservoir pathogen system to understand pathogen evolution and emergence.eLife · 2025Article
- Fungi Tryptophan Synthases: What Is the Role of the Linker Connecting the α and β Structural Domains inMolecules (Basel, Switzerland) · 2024Article
- Genome sequence ofMicrobiology resource announcements · 2023Article
- Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.FEMS microbiology reviews · 2023Article
- Gapless Genome Assembly of Puccinia triticina Provides Insights into Chromosome Evolution in Pucciniales.Microbiology spectrum · 2023Article
- Marker-Assisted Recurrent Selection for Pyramiding Leaf Rust and Coffee Berry Disease Resistance Alleles inGenes · 2023Article
- Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch PathogenPathogens (Basel, Switzerland) · 2022Article
- Current Status and Future Perspectives of Genomics Research in the Rust Fungi.International journal of molecular sciences · 2022Review
- Whole genome sequencing ofFrontiers in genetics · 2022Article
- Infection Strategies and Pathogenicity of Biotrophic Plant Fungal Pathogens.Frontiers in microbiology · 2022Review
- LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix.PloS one · 2022Article
- Evaluation of the Physicochemical Properties of Chitosans in Inducing the Defense Response ofPolymers · 2021Article
- Spatial and Temporal Genetic Diversity and Population Structure of Hemileia vastatrix from Peruvian Coffee Plantations.The plant pathology journal · 2021Article
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- The Conifer Root and Stem Rot Pathogen (Microorganisms · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.