Evidence map›Paper›PMID 30997289›Full record

ArticlePeerJ2019

Preparation of mitochondria to measure superoxide flashes in angiosperm flowers.

Chulan Zhang, Fengshuo Sun, Biao Xiong, Zhixiang Zhang

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Liproxstatin-1 Protects SH-SY5Y Cells by Inhibiting HInternational journal of molecular sciences · 2025
    Article
  2. Astaxanthin Inhibits HMarine drugs · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Chulan Zhang *College of Nature Conservation, Beijing Forestry University, Beijing, China.
Fengshuo Sun *College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
Biao XiongCollege of Tea Science, Guizhou University, Guizhou Province, China.
Zhixiang ZhangCollege of Nature Conservation, Beijing Forestry University, Beijing, China.
Beijing Forestry University · CNGuizhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMitochondria are the center of energy metabolism and the production of reactive oxygen species (ROS). ROS production results in a burst of "superoxide flashes", which is always accompanied by depolarization of mitochondrial membrane potential. Superoxide flashes have only been studied in the model plant

methodWe used 0.5 g samples to isolate mitochondria within <1.5 h from flowers of two non-transgenic plants (

resultsMitochondria isolated using our method showed a high respiration ratio. Our results indicate that the location of ROS and mitochondria was in a good coincidence. Increased ROS together with a higher frequency of superoxide flashes was found in mitochondria isolated from the flower pistil. Furthermore, a higher rate of depolarization of the ΔΨ m was observed in the pistil. Taken together, these results demonstrate that the frequency of superoxide flashes is closely related to depolarization of the ΔΨ m in petals and pistils of flowers.

Indexed as

Isolation of mitochondriaMitochondrial membrane potential (ΔΨm)ROSSuperoxide flashes

Identifiers

PMID30997289
PMCPMC6462395
OpenAlexW2937626373

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.