ArticleChemical science2019
Ionic polypeptide tags for protein phase separation.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 91 citations in OpenAlex.
- The Effect of Protein Tagging on Aggregation and Phase Separation.Journal of cellular biochemistry · 2026Review
- Reversible, Polymeric Complexation of Therapeutic Peptides Using Esterification.ACS macro letters · 2026Article
- Electrostatic Self-Assembly of Protein Nanoparticles from Intrinsically Disordered Polypeptide and Globular Protein Pairs.bioRxiv : the preprint server for biology · 2026Article
- Mass spectrometry integrates protein design into structural biology method development.QRB discovery · 2026Review
- Transient pH changes drive vacuole formation in enzyme-polymer condensates.Nature chemical engineering · 2026Article
- Design and applications of synthetic biomolecular condensates.Nature nanotechnology · 2026Review
- Cationic Amino Acid Identity and Net Charge Influence Condensate Properties inBiomacromolecules · 2025Article
- Exploring the effects of excipients on complex coacervation.Journal of colloid and interface science · 2025Article
- Adaptive peptide dispersions enable drying-induced biomolecule encapsulation.Nature materials · 2025Article
- Tandem-repeat proteins introduce tuneable properties to engineered biomolecular condensates.Chemical science · 2025Article
- Engineering Protein-Polyelectrolyte Interactions for Cellular Applications.Annual review of chemical and biomolecular engineering · 2025Review
- Spatiotemporal Control Over Protein Release from Artificial Cells via a Light-Activatable Protease.Advanced biology · 2025Article
- Recent progress of artificial cells in structure design, functionality and the prospects in food biotechnology.Materials today. Bio · 2025Review
- Genetically Engineered Liposwitch-Based Nanomaterials.Biomacromolecules · 2024Article
- Article
- Fluorescent protein tags affect the condensation properties of a phase-separating viral protein.Molecular biology of the cell · 2024Article
- Selectivity of Complex Coacervation in Multi-Protein Mixtures.bioRxiv : the preprint server for biology · 2024Article
- Sequestration of Small Ions and Weak Acids and Bases by a Polyelectrolyte Complex Studied by Simulation and Experiment.Macromolecules · 2024Article
- Design Rules for the Sequestration of Viruses into Polypeptide Complex Coacervates.Biomacromolecules · 2024Article
- Liquid-liquid phase separation driven by charge heterogeneity.Communications physics · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyelectrolytes of opposite charge in aqueous solution can undergo a liquid-liquid phase separation known as complex coacervation. Complex coacervation of ampholytic proteins with oppositely charged polyelectrolytes is of increasing interest as it results in a protein rich phase that has potential applications in protein therapeutics, protein purification, and biocatalysis. However, many globular proteins do not phase separate when mixed with an oppositely charged polyelectrolyte, and those that do phase separate do so over narrow concentration, pH, and ionic strength ranges. The protein design factors that govern complex coacervation under varying conditions are still relatively unexplored. Recent work indicates that proteins with an intrinsically disordered region, a higher net charge, or a patch of charged residues are more likely to undergo a phase transition. Based on these design parameters, polyionic coacervation tags were designed and assessed for their ability to promote protein complex coacervation with oppositely charged polyelectrolytes. The phase behavior of a panel of engineered proteins was evaluated with the strong polycation poly(4-vinyl
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.