ArticlePlant methods2019
Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 62 citations in OpenAlex.
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- Selection and Validation of Suitable Reference Genes for RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
- Physiological and transcriptional reprogramming for salinity tolerance of endangered mangrove associate Hernandia nymphaeifolia.BMC plant biology · 2025Article
- Validation of suitable reference microRNAs for qRT-PCR inFrontiers in plant science · 2025Article
- Genome-Wide Identification and Expression Profiling of Velvet Complex Transcription Factors inInternational journal of molecular sciences · 2024Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- Growth-regulating factor 15-mediated vascular cambium differentiation positively regulates wood formation in hybrid poplar (Frontiers in plant science · 2024Article
- A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.Forestry research · 2024Article
- MicroRNA miR171b Positively Regulates Resistance to Huanglongbing of Citrus.International journal of molecular sciences · 2023Article
- Integrative analysis of negatively regulated miRNA-mRNA axes for esophageal squamous cell carcinoma.Cancer biomarkers : section A of Disease markers · 2023Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of Nitraria sibirica under various abiotic stresses.BMC plant biology · 2022Article
- Identification of Putative Candidate Genes fromPlants (Basel, Switzerland) · 2022Article
- Identification of suitable reference genes for quantitative reverse transcription PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Analysis of aberrant miRNA-mRNA interaction networks in prostate cancer to conjecture its molecular mechanisms.Cancer biomarkers : section A of Disease markers · 2022Article
- Identification and validation of reference genes for qRT-PCR based studies in horse gram (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Efficient knockout of the phytoene desaturase gene in a hybrid poplar (Populus alba × Populus glandulosa) using the CRISPR/Cas9 system with a single gRNA.Transgenic research · 2021Article
- Identification of reliable reference genes for quantitative real-time PCR analysis of the Rhus chinensis Mill. leaf response to temperature changes.FEBS open bio · 2021Article
- Selection and Optimization of Reference Genes for MicroRNA Expression Normalization by qRT-PCR in Chinese Cedar (International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is a rapid and sensitive approach to identify miRNA and protein-coding gene expression in plants. However, because of the specially designated reverse transcription and shorter PCR products, very few reference genes have been identified for the quantitative analysis of miRNA expression in plants, and different internal reference genes are needed to normalize the expression of miRNAs and mRNA genes respectively. Therefore, it is particularly important to select the suitable common reference genes for normalization of quantitative PCR of miRNA and mRNA.
resultsIn this study, a modified reverse transcription PCR protocol was adopted for selecting and validating universal internal reference genes of mRNAs and miRNAs. Eight commonly used reference genes, four stably expressed novel genes in
conclusionsThe purpose of this study was to identify the most appropriate reference genes for qRT-PCR of miRNAs and mRNAs in different tissues at several developmental stages in poplar.
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