ArticleNature communications2019
Spatiotemporal control of coacervate formation within liposomes.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 218 citations in OpenAlex.
- Size of Biomolecular Condensates Dictates Fate in Liquid-Solid Phase Transitions through Amorphous-Amyloid Competition.Journal of the American Chemical Society · 2026Article
- Emerging Non-Conventional Approaches in mRNA-LNP Formulation for Therapeutic Applications.Pharmaceutics · 2026Review
- DNA affects the phenotype of fuel-dependent coacervate droplets.Nature communications · 2026Article
- Construction of Peptide Amphiphile-Coated Coacervates with Selective Permeability.ACS biomaterials science & engineering · 2026Article
- Spontaneous Fusion of Aqueous Two-Phase Droplets: Effect of Coexisting Phospholipids on Stability.Chembiochem : a European journal of chemical biology · 2025Article
- Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Active Armoring of Protocell Condensates with Metal-Phenolic Networks.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Article
- Surface Localized Coacervation Controlled by Bioactive Nanoarchitectonic Polyelectrolyte Multilayers.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Resilient Membranized Coacervates Formed through Spontaneous Wrapping of Heat-Destabilized Lipid Bilayers around Coacervate Droplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Spontaneous Formation of Core-Shell Microdroplets during Conventional Coacervate Phase Separation.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Adaptive ATP-induced molecular condensation in membranized protocells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Binary peptide coacervates as an active model for biomolecular condensates.Nature communications · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Organelle-like structural evolution of coacervate droplets induced by photopolymerization.Nature communications · 2025Article
- Network-forming phase separation of oppositely charged polyelectrolytes forming coacervates in a solvent.Nature communications · 2025Article
- Bio-Informed Porous Mineral-Based Composites.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Pyrite as a catalyst for the emergence of multiphase primitive cells.Frontiers in microbiology · 2025Article
- Enzymatic Reaction Network-Driven Polymerization-Induced Transient Coacervation.Angewandte Chemie (International ed. in English) · 2024Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation (LLPS), especially coacervation, plays a crucial role in cell biology, as it forms numerous membraneless organelles in cells. Coacervates play an indispensable role in regulating intracellular biochemistry, and their dysfunction is associated with several diseases. Understanding of the LLPS dynamics would greatly benefit from controlled in vitro assays that mimic cells. Here, we use a microfluidics-based methodology to form coacervates inside cell-sized (~10 µm) liposomes, allowing control over the dynamics. Protein-pore-mediated permeation of small molecules into liposomes triggers LLPS passively or via active mechanisms like enzymatic polymerization of nucleic acids. We demonstrate sequestration of proteins (FtsZ) and supramolecular assemblies (lipid vesicles), as well as the possibility to host metabolic reactions (β-galactosidase activity) inside coacervates. This coacervate-in-liposome platform provides a versatile tool to understand intracellular phase behavior, and these hybrid systems will allow engineering complex pathways to reconstitute cellular functions and facilitate bottom-up creation of synthetic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.