ArticleScientific reports2019
Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
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- Modeling metastasis - leveraging novel tools to streamline discovery in advanced cancer.Disease models & mechanisms · 2025Review
- Optimization of 3D Extrusion-Printed Particle-Containing Hydrogels for Osteogenic Differentiation.ACS omega · 2025Article
- Microfluidic Gastrointestinal Cell Culture Technologies-Improvements in the Past Decade.Biosensors · 2024Review
- Scaffold-based 3D cell culture models in cancer research.Journal of biomedical science · 2024Review
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid-Based 3D In Vitro Models.Advanced healthcare materials · 2023Review
- Griddient: a microfluidic array to generate reconfigurable gradients on-demand for spatial biology applications.Communications biology · 2023Article
- Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and metabolism.Biomaterials · 2023Article
- Simulating the Effect of Gut Microbiome on Cancer Cell Growth Using a Microfluidic Device.Sensors (Basel, Switzerland) · 2023Article
- Nano-liter perfusion microfluidic device made entirely by two-photon polymerization for dynamic cell culture with easy cell recovery.Scientific reports · 2023Article
- Enhanced growth and differentiation of neural stem cells on alginate/collagen/reduced graphene oxide composite hydrogel incorporated with lithium chloride.BioImpacts : BI · 2023Article
- Engineering Organ-on-a-Chip to Accelerate Translational Research.Micromachines · 2022Review
- Microphysiological model of renal cell carcinoma to inform anti-angiogenic therapy.Biomaterials · 2022Article
- On-chip perivascularLab on a chip · 2021Article
- Microfluidic tumor-on-a-chip model to evaluate the role of tumor environmental stress on NK cell exhaustion.Science advances · 2021Article
- Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis.Frontiers in cell and developmental biology · 2021Review
- Microfluidic Tumor-on-a-Chip Model to Study Tumor Metabolic Vulnerability.International journal of molecular sciences · 2020Article
- Hydrogels as artificial matrices for cell seeding in microfluidic devices.RSC advances · 2020Review
- Microfluidic lumen-based systems for advancing tubular organ modeling.Chemical Society reviews · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Microfluidics can be used to reproduce the TME in vitro and hence provide valuable insight on tumour evolution and drug sensitivity. However, microfluidics remains far from well-established mainstream molecular and cell biology methods. Therefore, we have developed a quick and straightforward collagenase-based enzymatic method to recover cells embedded in a 3D hydrogel in a microfluidic device with no impact on cell viability. We demonstrate the validity of this method on two different cell lines in a TME microfluidic model. Cells were successfully retrieved with high viability, and we characterised the different cell death mechanisms via AMNIS image cytometry in our model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.