ArticleProceedings of the National Academy of Sciences of the United States of America2019
Rapid screening of engineered microbial therapies in a 3D multicellular model.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Toward development of soil-derivedBiodesign research · 2026Article
- Burden-aware feedback control of microbial consortia.Nature communications · 2026Article
- Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition.FEBS open bio · 2026Article
- Bacterial therapies at the interface of synthetic biology and nanomedicine.Nature reviews bioengineering · 2024Article
- Dynamic Gene Expression Mitigates Mutational Escape in Lysis-Driven Bacteria Cancer Therapy.Biodesign research · 2024Article
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Antitrypanosomal Activity of 1,2,3-Triazole-Based Hybrids Evaluated Using In Vitro Preclinical Translational Models.Biology · 2023Article
- Steering Stem Cell Fate within 3D Living Composite Tissues Using Stimuli-Responsive Cell-Adhesive Micromaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Cross-talk between cancer and Pseudomonas aeruginosa mediates tumor suppression.Communications biology · 2023Article
- Article
- Design of combination therapy for engineered bacterial therapeutics in non-small cell lung cancer.Scientific reports · 2022Article
- A rapid screening platform to coculture bacteria within tumor spheroids.Nature protocols · 2022Review
- A programmable encapsulation system improves delivery of therapeutic bacteria in mice.Nature biotechnology · 2022Article
- Discovery and delivery strategies for engineered live biotherapeutic products.Trends in biotechnology · 2022Review
- Combating Infectious Diseases with Synthetic Biology.ACS synthetic biology · 2022Review
- Engineered Bacteria-Based Living Materials for Biotherapeutic Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Enhancing the tropism of bacteria via genetically programmed biosensors.Nature biomedical engineering · 2022Article
- Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments.ACS synthetic biology · 2021Article
- Theranostic cells: emerging clinical applications of synthetic biology.Nature reviews. Genetics · 2021Review
- In Vitro Miniaturized Tuberculosis Spheroid Model.Biomedicines · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Synthetic biology is transforming therapeutic paradigms by engineering living cells and microbes to intelligently sense and respond to diseases including inflammation, infections, metabolic disorders, and cancer. However, the ability to rapidly engineer new therapies far outpaces the throughput of animal-based testing regimes, creating a major bottleneck for clinical translation. In vitro approaches to address this challenge have been limited in scalability and broad applicability. Here, we present a bacteria-in-spheroid coculture (BSCC) platform that simultaneously tests host species, therapeutic payloads, and synthetic gene circuits of engineered bacteria within multicellular spheroids over a timescale of weeks. Long-term monitoring of bacterial dynamics and disease progression enables quantitative comparison of critical therapeutic parameters such as efficacy and biocontainment. Specifically, we screen
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.