ArticlePloS one2019
Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Microdroplet-based high-throughput screening for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi.Scientific reports · 2026Article
- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- Microfluidic droplet cultivation preserves microalgae diversity in screening systems.Scientific reports · 2025Article
- Ultrahigh-throughput screening of heterogeneous vancomycin-intermediate Staphylococcus aureus based on fluorescence-activated droplet sorting.BMC medicine · 2025Article
- Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria.Frontiers in microbiology · 2024Article
- Recent advances in droplet sequential monitoring methods for droplet sorting.Biomicrofluidics · 2023Review
- Droplet-Based Microfluidics: Applications in Pharmaceuticals.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Water-in-oil droplet-mediated method for detecting and isolating infectious bacteriophage particles via fluorescent staining.Frontiers in microbiology · 2023Article
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- 7-Aminocoumarin-4-acetic Acid as a Fluorescent Probe for Detecting Bacterial Dipeptidyl Peptidase Activities in Water-in-Oil Droplets and in Bulk.Analytical chemistry · 2022Article
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Emerging microfluidic technologies for microbiome research.Frontiers in microbiology · 2022Review
- Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort.Scientific reports · 2021Article
- Empowering a Methanol-DependentFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new method for selectively sorting droplets containing growing bacteria using a fluorescence resonance energy transfer (FRET)-based RNA probe. Bacteria and the FRET-based RNA probe are encapsulated into nanoliter-scale droplets, which are incubated to allow for cell growth. The FRET-based RNA probe is cleaved by RNase derived from the bacteria propagated in the droplets, resulting in an increase in fluorescence intensity. The fluorescent droplets containing growing bacteria are distinguishable from quenching droplets, which contain no cells. We named this method FNAP-sort based on the use of a fluorescent nucleic acid probe in droplets for bacterial sorting. Droplets containing the FRET-based RNA probe and four species of pure cultures, which grew in the droplets, were selectively enriched on the basis of fluorescence emission. Furthermore, fluorescent droplets were sorted from more than 500,000 droplets generated using environmental soil bacteria and the FRET-based RNA probe on days 1, 3, and 7 with repeated incubation and sorting. The bacterial compositions of sorted droplets differed on days 1, 3, and 7; moreover, on day 7, the bacterial composition of the fluorescent droplets was drastically different from that of the quenching droplets. We believe that FNAP-sort is useful for high-throughput cultivation and sorting of environmental samples containing bacteria with various growth rates, including slow-growing microbes that require long incubation times.
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