Evidence map›Paper›PMID 30992318›Full record

ArticleG3 (Bethesda, Md.)2019

Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the

Adam Hefel, Sarit Smolikove

Open access · goldAbstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. WormTagDB: A Systematic Survey of Endogenously Tagged Proteins inbioRxiv : the preprint server for biology · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags inProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  11. Article
  12. Article
  13. Aging Negatively Impacts DNA Repair and Bivalent Formation in theFrontiers in cell and developmental biology · 2021
    Article
  14. Review
  15. Article
  16. TheGenetics · 2019
    Review
  17. Synaptic remodeling, lessons fromJournal of neurogenetics
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Adam HefelDepartment of Biology, University of Iowa, Iowa City, IA 52240.ORCID 0000-0002-2135-0152
Sarit SmolikoveDepartment of Biology, University of Iowa, Iowa City, IA 52240 sarit-smolikove@uiowa.edu.ORCID 0000-0003-0062-3606
University of Iowa · US

Funding

An organismal approach to dissecting the mechanism of DSB repair pathway choiceR01GM112657 · NIGMS · UNIVERSITY OF IOWA · PI SMOLIKOVE, SARIT · 2015 to 2019
$1.5M
NIGMS NIH HHS R01 GM112657
6 · The paper itself

Abstract

Identifying protein localization is a useful tool in analyzing protein function. Using GFP-fusion tags, researchers can study the function of endogenous proteins in living tissue. However, these tags are considerably large, making them difficult to insert, and they can potentially affect the normal function of these proteins. To improve on these drawbacks, we have adopted the split sfGFP system for studying the localization of proteins in the

Indexed as

AnimalsBiomarkersCaenorhabditis elegansCaenorhabditis elegans ProteinsCloning, MolecularFertilityGene ExpressionGerm CellsGreen Fluorescent ProteinsMolecular ImagingRecombinant Fusion ProteinsBiomarkersCaenorhabditis elegans ProteinsGreen Fluorescent ProteinsRecombinant Fusion ProteinsAKIR-1CRISPRgermlineGFP11GFP1-10meiosisRPA-1sfGFPsplit GFPSYP-4

Identifiers

PMID30992318
PMCPMC6553534
OpenAlexW2937129423

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.