ArticleMalaria journal2019
Sequence-based identification of Anopheles species in eastern Ethiopia.
Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Systematic review of sporozoite infection rate of Anopheles mosquitoes in Ethiopia, 2001-2021.Parasites & vectors · 2023Pooled it
- Bionomic characterization of Anopheles mosquitoes in the Ethiopian highlands and lowlands.Parasites & vectors · 2024Article
- A decade of invasive Anopheles stephensi sequence-based identification: toward a global standard.Trends in parasitology · 2024Review
- Identification of breeding habitats and kdr mutations in Anopheles spp. in South Korea.Malaria journal · 2023Article
- First report of Anopheles stephensi from southern Ethiopia.Malaria journal · 2023Article
- PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras.Malaria journal · 2023Article
- Genetic diversity, phylogenetic and phylogeographic analysis of Anopheles culicifacies species complex using ITS2 and COI sequences.PloS one · 2023Article
- Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes.Parasites & vectors · 2022Article
- Morphological identification and genetic characterization of Anopheles stephensi in Somaliland.Parasites & vectors · 2022Article
- Analysis of the Knockdown Resistance Locus (kdr) in Anopheles stephensi, An. arabiensis, and Culex pipiens s.l. for Insight Into the Evolution of Target-site Pyrethroid Resistance in Eastern Ethiopia.The American journal of tropical medicine and hygiene · 2022Article
- Temporally consistent predominance and distribution of secondary malaria vectors in the Anopheles community of the upper Zambezi floodplain.Scientific reports · 2022Article
- Genetic diversity of Anopheles stephensi in Ethiopia provides insight into patterns of spread.Parasites & vectors · 2021Article
- Development of a chimeric odour blend for attracting gravid malaria vectors.Malaria journal · 2021Article
- New vectors that are early feeders for Plasmodium knowlesi and other simian malaria parasites in Sarawak, Malaysian Borneo.Scientific reports · 2021Article
- Extensive new Anopheles cryptic species involved in human malaria transmission in western Kenya.Scientific reports · 2020Article
- Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting.Parasites & vectors · 2020Article
- Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni.Malaria journal · 2020Article
- Geographical distribution of Anopheles stephensi in eastern Ethiopia.Parasites & vectors · 2020Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe recent finding of a typically non-African Anopheles species in eastern Ethiopia emphasizes the need for detailed species identification and characterization for effective malaria vector surveillance. Molecular approaches increase the accuracy and interoperability of vector surveillance data. To develop effective molecular assays for Anopheles identification, it is important to evaluate different genetic loci for the ability to characterize species and population level variation. Here the utility of the internal transcribed spacer 2 (ITS2) and cytochrome oxidase I (COI) loci for detection of Anopheles species from understudied regions of eastern Ethiopia was investigated.
methodsAdult mosquitoes were collected from the Harewe locality (east) and Meki (east central) Ethiopia. PCR and Sanger sequencing were performed for portions of the ITS2 and COI loci. Both NCBI's Basic Local Alignment Search tool (BLAST) and phylogenetic analysis using a maximum-likelihood approach were performed to identify species of Anopheles specimens.
resultsTwo species from the east Ethiopian collection, Anopheles arabiensis and Anopheles pretoriensis were identified. Analyses of ITS2 locus resulted in delineation of both species. In contrast, analysis of COI locus could not be used to delineate An. arabiensis from other taxa in Anopheles gambiae complex, but could distinguish An. pretoriensis sequences from sister taxa.
conclusionThe lack of clarity from COI sequence analysis highlights potential challenges of species identification within species complexes. These results provide supporting data for the development of molecular assays for delineation of Anopheles in east Ethiopia.
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