ArticleBMC plant biology2019
Identification of transcription factors of nitrate reductase gene promoters and NRE2 cis-element through yeast one-hybrid screening in Nicotiana tabacum.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Endogenous Plant Nitrous Oxide Formation: Evidence, Mechanisms and Ecosystem Implications.Plants (Basel, Switzerland) · 2026Review
- Regulation and functional analysis of BvMBF1c in response to Cercospora leaf spot (CLS) stresses in sugar beet (Beta vulgaris L.).BMC plant biology · 2025Article
- Identification and expression characteristics of NIN-like protein (NLP) gene family in cucumber plant (Cucumis sativus L.).BMC plant biology · 2025Article
- StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity.The New phytologist · 2025Article
- CDPK protein in cotton: genomic-wide identification, expression analysis, and conferring resistance to heat stress.BMC plant biology · 2024Article
- TheInternational journal of molecular sciences · 2024Article
- Identification of Transcription Factors of Santalene Synthase Gene Promoters andPlants (Basel, Switzerland) · 2024Article
- Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum.BMC genomics · 2022Article
- Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco.BMC plant biology · 2022Article
- Construction of yeast one-hybrid library and screening of transcription factors regulating LhMYBSPLATTER expression in Asiatic hybrid lilies (Lilium spp.).BMC plant biology · 2021Article
- Functional Analysis of Wheat NAC Transcription Factor,Frontiers in plant science · 2021Article
- Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays.BMC biotechnology · 2020Article
- Tissue-specificPlant signaling & behavior · 2019Article
- Decoding complexity: tackling the challenge of how many transcription factors regulate a plant gene.TranscriptionReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThis study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2. Based on the constructed cDNA library of Nicotiana tabacum K326, a yeast one-hybrid system was established using the Matchmaker® Gold Yeast One-Hybrid Library Screening System from Clontech. The transcription factors of NIA1 andNIA2 promoters and NRE2 cis-elements were screened.
resultsAfter sequencing and bioinformatics analysis, 15 cDNA sequences were identified: 9 for NIA1 (including XP_016503563.1 and NP_001312236.1), 3 for NIA2 (including XP_016510250.1), and 3 for NRE2 (including XM_016576899.1). XP_016503563.1 was annotated in PREDICTED: CRM-domain containing factor CFM3, and NP_001312236.1chloroplastic/mitochondrial-like in Nicotiana tabacum. NP_001312236.1 was annotated in Sulfite oxidase-like of Nicotiana tabacum. XP_016510250.1 was annotated as PREDICTED: uncharacterized protein LOC107827596 in Nicotiana tabacum. XM_016576899.1 was annotated in PREDICTED: Nicotiana tabacum RING-H2 finger protein ATL16-like (LOC107759033).
conclusionA yeast one-hybrid library was successfully constructed. The identified transcription factors may provide a theoretical basis for the study of plant nitrate reductase.
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