ArticleNature protocols2019
High-speed imaging of glutamate release with genetically encoded sensors.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Optical measurement of glutamate release robustly reports short-term plasticity at a fast central synapse.Frontiers in molecular neuroscience · 2024Article
- Article
- Extrasynaptic NMDA receptors in acute and chronic excitotoxicity: implications for preventive treatments of ischemic stroke and late-onset Alzheimer's disease.Molecular neurodegeneration · 2023Review
- Article
- Vesicular release probability sets the strength of individual Schaffer collateral synapses.Nature communications · 2022Article
- Optical analysis of glutamate spread in the neuropil.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Article
- Asynchronous glutamate release is enhanced in low release efficacy synapses and dispersed across the active zone.Nature communications · 2022Article
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Population imaging of synaptically released glutamate in mouse hippocampal slices.STAR protocols · 2021Article
- Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons.PLoS biology · 2021Article
- Building a circuit through correlated spontaneous neuronal activity in the developing vertebrate and invertebrate visual systems.Genes & development · 2021Review
- A modified fluorescent sensor for reporting glucose concentration in the airway lumen.PloS one · 2021Article
- Freeze-frame imaging of synaptic activity using SynTagMA.Nature communications · 2020Article
- Simultaneous Quantification of Vesicle Size and Catecholamine Content by Resistive Pulses in Nanopores and Vesicle Impact Electrochemical Cytometry.Journal of the American Chemical Society · 2020Article
- A Practical Guide to Using CV Analysis for Determining the Locus of Synaptic Plasticity.Frontiers in synaptic neuroscience · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The strength of an excitatory synapse depends on its ability to release glutamate and on the density of postsynaptic receptors. Genetically encoded glutamate indicators (GEGIs) allow eavesdropping on synaptic transmission at the level of cleft glutamate to investigate properties of the release machinery in detail. Based on the sensor iGluSnFR, we recently developed accelerated versions of GEGIs that allow investigation of synaptic release during 100-Hz trains. Here, we describe the detailed procedures for design and characterization of fast iGluSnFR variants in vitro, transfection of pyramidal cells in organotypic hippocampal cultures, and imaging of evoked glutamate transients with two-photon laser-scanning microscopy. As the released glutamate spreads from a point source-the fusing vesicle-it is possible to localize the vesicle fusion site with a precision exceeding the optical resolution of the microscope. By using a spiral scan path, the temporal resolution can be increased to 1 kHz to capture the peak amplitude of fast iGluSnFR transients. The typical time frame for these experiments is 30 min per synapse.
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Registered trials
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