ArticleScientific reports2019
Release of eDNA by different life history stages and during spawning activities of laboratory-reared Japanese eels for interpretation of oceanic survey data.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Beyond Exponential Decay: How Biphasic and Delayed Decay Dynamics Shape Marine eDNA Dispersal.Ecology and evolution · 2026Article
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- Applications of environmental DNA monitoring for seaweed reproductive phenology: A case study with giant kelp (Macrocystis pyrifera).Journal of phycology · 2025Article
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- Optimizing detectability of the endangered fan mussel using eDNA and ddPCR.Ecology and evolution · 2024Article
- Article
- Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.Scientific reports · 2022Article
- Meta-analysis shows that environmental DNA outperforms traditional surveys, but warrants better reporting standards.Ecology and evolution · 2021Article
- Environmental (e)RNA advances the reliability of eDNA by predicting its age.Scientific reports · 2021Article
- Development of droplet digital Polymerase Chain Reaction assays for the detection of long-finned (PeerJ · 2021Article
- Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel.PloS one · 2021Article
- Field application of an improved protocol for environmental DNA extraction, purification, and measurement using Sterivex filter.Scientific reports · 2020Article
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- Estimations of Riverine Distribution, Abundance, and Biomass of Anguillid Eels in Japan and Taiwan Using Environmental DNA Analysis.Zoological studies · 2020Article
- Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander (PeerJ · 2020Article
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11 authors.
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Abstract
To assist in detection of offshore spawning activities of the Japanese eel Anguilla japonica and facilitate interpretation of results of environmental DNA (eDNA) analysis in their spawning area, we examined the eDNA concentration released by each life history stage of artificially reared Japanese eels in the laboratory using quantitative real-time PCR (qPCR). We also compared eDNA concentrations between before and after artificially induced spawning activities. eDNA was not detected from three 30 L seawater tanks containing each single fertilized egg, but eDNA was found from other tanks each containing single individuals of larval stages (preleptocephalus and leptocephalus), juvenile stages (glass eel, elver and yellow eel) or adult stage (silver eel). The eDNA concentrations increased in the life history stages, showed a significant difference among all stages, and were positively correlated with the total length and wet weight. Moreover, the eDNA concentration after spawning was 10-200 times higher than that before spawning, which indicated that the spawning events in the ocean would produce relatively high eDNA concentration. These results in the laboratory suggested that eDNA analysis appears to be an effective method for assisting oceanic surveys to estimate the presence and spawning events of the Japanese eel in the spawning area.
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