Evidence map›Paper›PMID 30988274›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2019

Identification of Altered Circular RNA Expression in Serum Exosomes from Patients with Papillary Thyroid Carcinoma by High-Throughput Sequencing.

Chunjiang Yang, Youchun Wei, Leitao Yu, Yong Xiao

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In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Circular RNAs: characteristics, functions, mechanisms, and potential applications in thyroid cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2024
    Review
  9. Review
  10. Review
  11. Review
  12. CTC, ctDNA, and Exosome in Thyroid Cancers: A Review.International journal of molecular sciences · 2023
    Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?International journal of molecular sciences · 2022
    Article
  18. Review
  19. Article
  20. CircRNA: A novel potential strategy to treat thyroid cancer (Review).International journal of molecular medicine · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chunjiang YangDepartment of Thyroid Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
Youchun WeiDepartment of Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
Leitao YuDepartment of Thyroid Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
Yong XiaoGraduate School of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND This study aimed to identify altered exosome circular RNA (circRNA) in the serum of patients with papillary thyroid carcinoma using high-throughput sequencing. MATERIAL AND METHODS Serum was collected from three patients with papillary thyroid carcinoma and three patients with a benign thyroid goiter. Exosomes were isolated using an exosome isolation kit and confirmed by transmission electron microscopy. Exosome circRNAs were analyzed by high-throughput sequencing using the HiSeq 4000 sequencer. The differentially expressed circRNAs were confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS Twenty-two differentially expressed circRNAs were screened, which included three that were upregulated and 19 that were down-regulated in serum from patients with papillary thyroid carcinoma compared with controls. Gene Ontology (GO) enrichment analysis showed that these differentially expressed circRNAs were associated with 16 signaling pathways, including the thyroid hormone signaling pathway, the PI3K-Akt signaling pathway, and the AMPK signaling pathway. Three differentially regulated circRNAs included hsacirc_007293, hsacirc_031752, and hsacirc_020135 were confirmed by qRT- PCR. The expression trends were consistent between the high-throughput sequencing technique and qRT-PCR. CONCLUSIONS The findings from this study have shown that gene regulation can be studied from exosomes obtained from serum samples in patients with papillary thyroid carcinoma, and supports the need for further studies on the role of exosome circRNAs in thyroid cancer.

Indexed as

AdultComputational BiologyExosome Multienzyme Ribonuclease ComplexExosomesFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticHigh-Throughput Nucleotide SequencingHumansMiddle AgedOligonucleotide Array Sequence AnalysisReal-Time Polymerase Chain ReactionRNARNA, CircularThyroid Cancer, PapillaryThyroid NeoplasmsExosome Multienzyme Ribonuclease ComplexRNARNA, Circular

Identifiers

PMID30988274
PMCPMC6481237

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.