Complete mapping of disulfide linkages for etanercept products by multi-enzyme digestion coupled with LC-MS/MS using multi-fragmentations including CID and ETD.

Li-Juan Huang, Chia-Wang Chiang, Shun-Li Chen, Shih-Yao Wei, Shu-Hui Chen

Open access · hybridAbstract read

In one paragraph

Article in Journal of food and drug analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.5field-weighted citation impact, top 37% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 12 citations in OpenAlex.

Instability Challenges and Stabilization Strategies of Pharmaceutical Proteins.Pharmaceutics · 2022
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Li-Juan HuangBio-Analytical Lab, Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.

Chia-Wang ChiangPharamceutical Analysis Department, Mycenax Biotech Inc., Taiwan.

Shun-Li ChenBio-Analytical Lab, Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.

Shih-Yao WeiBio-Analytical Lab, Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.

Shu-Hui ChenBio-Analytical Lab, Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.

National Cheng Kung University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The disulfide linkages of two etanercept products, Enbrel® (innovator drug) and TuNEX®, were characterized and compared using a multi-fragmentation approach consisting of electron transfer dissociation (ETD) and collision induced dissociation (CID) in combination with multi-enzyme digestion protocols (from Lys-C, trypsin, Glu-C, and PNGase F). Multi-fragmentation approach allowed multi-disulfide linkages contained in a peptide to be un-ambiguously assigned based on the cleavage of both the disulfide and the backbone linkages in a MS

Indexed as

Chromatography, LiquidDisulfidesElectron TransportEtanerceptHumansMetalloendopeptidasesPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidaseSerine EndopeptidasesTandem Mass SpectrometryTrypsinDisulfidesEtanerceptglutamyl endopeptidaseMetalloendopeptidasesPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidasepeptidyl-Lys metalloendopeptidaseSerine EndopeptidasesTrypsinCIDEnbrel®ETDLC-MSTuNEX®

Identifiers

PMID30987725

DOI10.1016/j.jfda.2018.11.007

PMCPMC9296193

OpenAlexW2906923598

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.