ArticleViruses2019
Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Unlocking translational control of specialized metabolism in plants through 5'UTR structure.Science advances · 2026Article
- Reorganizing the RNA polymerase II complex for replication of an infectious noncoding RNA in vivo.PLoS pathogens · 2026Article
- Evolutionarily New Solanaceous DCL2 Family Members Acquire Functions in Tomato.Molecular plant pathology · 2025Article
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- A nuclear import pathway exploited by pathogenic noncoding RNAs.The Plant cell · 2022Article
- Heparin-Loaded Alginate Hydrogels: Characterization and Molecular Mechanisms of Their Angiogenic and Anti-Microbial Potential.Materials (Basel, Switzerland) · 2022Article
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant-viroid interactions represent a valuable model for delineating structure-function relationships of noncoding RNAs. For various functional studies, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on TRI Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantities, such as protoplasts. Given the ease and the robustness of this new method, it will have broad applications in virology and other disciplines in molecular biology.
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Registered trials
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