Evidence map›Paper›PMID 30986357›Full record

ArticleBiochemistry and cell biology = Biochimie et biologie cellulaire2019

Effect of caveolin-1 on Stat3-ptyr705 levels in breast and lung carcinoma cells.

Mulu Geletu, Zaid Taha, Rozanne Arulanandam, Reva Mohan, Hikmat H Assi, Maria G Castro, Ivan Robert Nabi, Patrick T Gunning, Leda Raptis

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and cell biology = Biochimie et biologie cellulaire, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Mulu GeletuDepartment of Biomedical and Molecular Sciences, Pathology and Molecular Medicine, and Queen's University Cancer Research Institute, Queen's University, Kingston, ON K7L 3N6, Canada.
Zaid TahaOttawa Hospital Research Institute - Cancer Therapeutics, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada.
Rozanne ArulanandamOttawa Hospital Research Institute - Cancer Therapeutics, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada.
Reva MohanDepartment of Biomedical and Molecular Sciences, Pathology and Molecular Medicine, and Queen's University Cancer Research Institute, Queen's University, Kingston, ON K7L 3N6, Canada.
Hikmat H AssiDepartment of Neurosurgery and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48019, USA.
Maria G CastroDepartment of Neurosurgery and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48019, USA.
Ivan Robert NabiDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Patrick T GunningDepartment of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada.
Leda RaptisDepartment of Biomedical and Molecular Sciences, Pathology and Molecular Medicine, and Queen's University Cancer Research Institute, Queen's University, Kingston, ON K7L 3N6, Canada.
University of Toronto · CAUniversity of British Columbia · CA

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI THEODORE S LAWRENCE · 1988 to 2026
$178.2M
Immune-suppressive Myeloid Cells in the Glioma Microenvironment: Signaling Mechanisms and Novel Therapeutic StrategiesR37NS094804 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CASTRO, MARIA G · 2015 to 2021
$2.9M
Novel combined immunotherapeutic strategies for glioma: using pet dogs as a large animal spontaneous modelU01CA224160 · NCI · UNIVERSITY OF MINNESOTA · PI PLUHAR, GRACE ELIZABETH · 2017 to 2021
$2.7M
Interactions between the tumor cells and the neuro-immune microenvironment in mutant IDH1 gliomas: implications for therapeuticsR01NS105556 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CASTRO, MARIA G · 2018 to 2021
$1.9M
CIHR PJT-148698NCI NIH HHS P30 CA046592NCI NIH HHS U01 CA224160NINDS NIH HHS R01 NS105556NINDS NIH HHS R37 NS094804
6 · The paper itself

Abstract

We recently demonstrated that Cav1 (caveolin-1) is a negative regulator of Stat3 (signal transducer and activator of transcription-3) activity in mouse fibroblasts and human lung carcinoma SHP77 cells. We now examined whether the cellular context may affect their levels as well as the relationship between them, by assessing Cav1 and Stat3-ptyr705 amounts in different cell lines. In MDA-MB-231, A549, and HaCat cells, Cav1 levels were high and Stat3-ptyr705 levels were low, consistent with the notion of a negative effect of endogenous Cav1 on Stat3-ptyr705 levels in these lines. In addition, manipulation of Cav1 levels revealed a negative effect in MCF7 and mouse fibroblast cells, while Cav1 upregulation induced apoptosis in MCF7 cells. In contrast, however, line MRC9 had high Cav1 and high Stat3-ptyr705 levels, indicating that high Cav1 is insufficient to reduce Stat3-ptyr705 levels in this line. MCF7 and LuCi6 cells had very low Cav1 and Stat3-ptyr705 levels, indicating that the low Stat3-ptyr705 can be independent from Cav1 levels altogether. Our results reveal a further level of complexity in the relationship between Cav1 and Stat3-ptyr705 than previously thought. In addition, we demonstrate that in a feedback loop, Stat3 inhibition upregulates Cav1 in HeLa cells but not in other lines tested.

Indexed as

AnimalsBreast NeoplasmsCaveolin 1Cells, CulturedFemaleHumansLung NeoplasmsMiceMice, Inbred BALB CSTAT3 Transcription FactorTyrosineCAV1 protein, humanCaveolin 1STAT3 protein, humanSTAT3 Transcription FactorTyrosinecancercaveolin-1cavéoline-1cell lineslignées cellulairesStat3

Identifiers

PMID30986357
PMCPMC7038897
OpenAlexW2939678044

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.