ArticlePloS one2019
Accessory genome of the multi-drug resistant ocular isolate of Pseudomonas aeruginosa PA34.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Prioritization of Critical Factors for Surveillance of the Dissemination of Antibiotic Resistance inInternational journal of molecular sciences · 2023Pooled it
- Comparative Genomic Analysis ofInfection and drug resistance · 2026Article
- Whole genome sequencing and molecular characterization of twoFrontiers in bioinformatics · 2026Article
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2025Review
- Model-driven characterization of functional diversity ofMicrobial genomics · 2024Article
- Review
- Comparative Genomic Analyses of Virulence and Antimicrobial Resistance inMicroorganisms · 2023Article
- Comparative Genomics ofAntibiotics (Basel, Switzerland) · 2023Article
- Application of prophage sequence analysis to investigate a disease outbreak involvingFrontiers in microbiology · 2023Article
- Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review.International journal of ophthalmology · 2022Review
- Whole Genome Multi-Locus Sequence Typing and Genomic Single Nucleotide Polymorphism Analysis for Epidemiological Typing ofFrontiers in microbiology · 2022Article
- CRISPR-Cas systems restrict horizontal gene transfer in Pseudomonas aeruginosa.The ISME journal · 2021Article
- Estimation of pathogenic potential of an environmental Pseudomonas aeruginosa isolate using comparative genomics.Scientific reports · 2021Article
- The Basis for Natural Multiresistance to Phage inAntibiotics (Basel, Switzerland) · 2020Article
- A Core Genome Multilocus Sequence Typing Scheme forFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria can acquire an accessory genome through the horizontal transfer of genetic elements from non-parental lineages. This leads to rapid genetic evolution allowing traits such as antibiotic resistance and virulence to spread through bacterial communities. The study of complete genomes of bacterial strains helps to understand the genomic traits associated with virulence and antibiotic resistance. We aimed to investigate the complete accessory genome of an ocular isolate of Pseudomonas aeruginosa strain PA34. We obtained the complete genome of PA34 utilising genome sequence reads from Illumina and Oxford Nanopore Technology followed by PCR to close any identified gaps. In-depth genomic analysis was performed using various bioinformatics tools. The susceptibility to heavy metals and cytotoxicity was determined to confirm expression of certain traits. The complete genome of PA34 includes a chromosome of 6.8 Mbp and two plasmids of 95.4 Kbp (pMKPA34-1) and 26.8 Kbp (pMKPA34-2). PA34 had a large accessory genome of 1,213 genes and had 543 unique genes not present in other strains. These exclusive genes encoded features related to metal and antibiotic resistance, phage integrase and transposons. At least 24 genomic islands (GIs) were predicated in the complete chromosome, of which two were integrated into novel sites. Eleven GIs carried virulence factors or replaced pathogenic genes. A bacteriophage carried the aminoglycoside resistance gene (AAC(3)-IId). The two plasmids carried other six antibiotic resistance genes. The large accessory genome of this ocular isolate plays a large role in shaping its virulence and antibiotic resistance.
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