ArticleOptica2018
High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy.
Article in Optica, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed.
- Label-free correlative morpho-chemical tomography of 3D kidney mesangial cells.Journal of biomedical optics · 2026Article
- Real-time, high-resolution metabolic characterization of live bacteria using label-free optical metabolic imaging.NPJ biofilms and microbiomes · 2026Article
- Artificial Intelligence in Label-Free Optical Imaging Applications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Non-invasive Imaging Techniques: From Histology to In-vivo Imaging.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- A low-cost FPGA-based approach for pile-up corrected high-speedNeurophotonics · 2025Article
- In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.Journal of biomedical optics · 2024Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- Developing 1,4-Diethyl-1,2,3,4-tetrahydroquinoxalin-substituted Fluorogens Based on GFP Chromophore for Endoplasmic Reticulum and Lysosome Staining.International journal of molecular sciences · 2024Article
- Enhancing Therapeutic Response and Overcoming Resistance to Checkpoint Inhibitors in Ovarian Cancer through Cell Cycle Regulation.International journal of molecular sciences · 2024Article
- Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D.Current opinion in solid state & materials science · 2024Article
- From Cell Populations to Molecular Complexes: Multiplexed Multimodal Microscopy to Explore p53-53BP1 Molecular Interaction.International journal of molecular sciences · 2024Article
- Analog multiplexing of a laser clock and computational photon counting for fast fluorescence lifetime imaging microscopy.Biomedical optics express · 2024Article
- Article
- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Label-free biomedical optical imaging.Nature photonics · 2023Article
- Near-Infrared Fluorescence Imaging in Preclinical Models of Glioblastoma.Journal of imaging · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of autofluorescent metabolic coenzymes has been widely used to investigate energetic perturbations in living cells and tissues in a label-free manner with subcellular resolution. While the currently used state-of-the-art instruments are highly sensitive to local molecular changes associated with these metabolic processes, they are inherently slow and limit the study of dynamic metabolic environments. Here, a sustained video-rate 2P-FLIM imaging system is demonstrated for time-lapse lifetime imaging of reduced nicotinamide adenine dinucleotide, an autofluorescent metabolic coenzyme involved in both aerobic and anaerobic processes. This system is sufficiently sensitive to differences in metabolic activity between aggressive and nonaggressive cancer cell lines and is demonstrated for both wide field-of-view autofluorescence imaging as well as sustained video-rate image acquisition of metabolic dynamics following induction of apoptosis. The unique capabilities ofthis imaging platform provide a powerful technological advance to further explore rapid metabolic dynamics in living cells.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.