ArticleFrontiers in bioengineering and biotechnology2019
Analysis of Mechanically Activated Ion Channels at the Cell-Substrate Interface: Combining Pillar Arrays and Whole-Cell Patch-Clamp.
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Mechanosensitive Ion Channels: The Unending Riddle of Mechanotransduction.Bioelectricity · 2025Review
- High-throughput virtual search of small molecules for controlling the mechanical stability of human CD4.The Journal of biological chemistry · 2024Article
- TMEM120A/TACAN: A putative regulator of ion channels, mechanosensation, and lipid metabolism.Channels (Austin, Tex.) · 2023Review
- Force From Filaments: The Role of the Cytoskeleton and Extracellular Matrix in the Gating of Mechanosensitive Channels.Frontiers in cell and developmental biology · 2022Review
- Ion channel mediated mechanotransduction in immune cells.Current opinion in solid state & materials science · 2021Article
- Functional Expression of Piezo1 in Dorsal Root Ganglion (DRG) Neurons.International journal of molecular sciences · 2020Article
- Article
- Modulating the Mechanical Activation of TRPV4 at the Cell-Substrate Interface.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ionic currents can be evoked by mechanical inputs applied directly at the cell-substrate interface. These ionic currents are mediated by mechanically activated ion channels, where the open probability increases with increasing mechanical input. In order to study mechanically activated ion channels directly at the interface between cells and their environment, we have developed a technique to simultaneously monitor ion channel activity whilst stimuli are applied via displacement of cell-substrate contacts. This technique utilizes whole-cell patch-clamp electrophysiology and elastomeric pillar arrays, it is quantitative and appropriate for studying channels that respond to stimuli that are propagated to an adherent cell via the physical substrate. The mammalian channels PIEZO1, PIEZO2 have been shown to be activated by substrate deflections, using this technique. In addition, TRPV4 mediated currents can be evoked by substrate deflections, in contrast to alternate stimulation methods such as membrane stretch or cellular indentation. The deflections applied at cell-substrate points mimic the magnitude of physical stimuli that impact cells
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