ArticleMicrobiome2019
Encrustations on ureteral stents from patients without urinary tract infection reveal distinct urotypes and a low bacterial load.
Article in Microbiome, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02845726 (Biofilms on Short-term Indwelling Ureteral Stents), which is not on this map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Biofilms on Short-term Indwelling Ureteral Stents: Influence on Associated Symptoms and Complications; Prediction by Urine Analysis; Current Bacterial Spectrum and Antibiotic Resistance
Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Quantitative analysis and risk factors of high-grade encrustation in double-J stents after ureteroscopy and laser lithotripsy.Urolithiasis · 2026Article
- Evaluation of an Antibiotic Strategy for Ureteral Stent Removal After Kidney Transplant.European urology open science · 2025Article
- Revealing the potential value of urine microbiota in ureteral stent encrustation after renal transplantation via Illumina sequencing of 16S rRNA genes.BMC microbiology · 2025Article
- Pearl in a seashell: epitaxy of solitary cystolith on the leftover curl of a double j stent - a case report.Annals of medicine and surgery (2012) · 2025Article
- Comparison of the microbiome of bladder urine, upper urinary tract urine, and kidney stones in patients with urolithiasis.Central European journal of urology · 2025Article
- Unraveling the impact of host genetics and factors on the urinary microbiome in a young population.mBio · 2024Article
- The Microbiome's Influence on Head and Neck Cancers.Current oncology reports · 2023Review
- Identifying ureteral stent encrustation using machine learning based on CT radiomics features: a bicentric study.Frontiers in medicine · 2023Article
- The interplay between bacterial biofilms, encrustation, and wall shear stress in ureteral stents: a review across scales.Frontiers in urology · 2023Review
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- A preliminary study on the role of Bacteroides fragilis in stent encrustation.World journal of urology · 2021Article
- Surface analysis of ureteral stent before and after implantation in the bodies of child patients.Urolithiasis · 2021Article
- Problems and solutions of stent biofilm and encrustations: A review of literature.Turkish journal of urology · 2020Review
- Ureteral Stent Microbiota Is Associated with Patient Comorbidities but Not Antibiotic Exposure.Cell reports. Medicine · 2020Article
- Oligosaccharides and Complex Carbohydrates: A New Paradigm for Cranberry Bioactivity.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent knowledge of the urinary tract microbiome is limited to urine analysis and analysis of biofilms formed on Foley catheters. Bacterial biofilms on ureteral stents have rarely been investigated, and no cultivation-independent data are available on the microbiome of the encrustations on the stents.
resultsThe typical encrustations of organic and inorganic urine-derived material, including microbial biofilms formed during 3-6 weeks on ureteral stents in patients treated for kidney and ureteral stones, and without reported urinary tract infection at the time of stent insertion, were analysed. Next-generation sequencing of the 16S rRNA gene V3-V4 region revealed presence of different urotypes, distinct bacterial communities. Analysis of bacterial load was performed by combining quantification of 16S rRNA gene copy numbers by qPCR with microscopy and cultivation-dependent analysis methods, which revealed that ureteral stent biofilms mostly contain low numbers of bacteria. Fluorescence microscopy indicates the presence of extracellular DNA. Bacteria identified in biofilms by microscopy had mostly morphogenic similarities to gram-positive bacteria, in few cases to Lactobacillus and Corynebacterium, while sequencing showed many additional bacterial genera. Weddellite crystals were absent in biofilms of patients with Enterobacterales and Corynebacterium-dominated microbiomes.
conclusionsThis study provides novel insights into the bacterial burden in ureteral stent encrustations and the urinary tract microbiome. Short-term (3-6 weeks) ureteral stenting is associated with a low load of viable and visible bacteria in ureteral stent encrustations, which may be different from long-term stenting. Patients could be classified according to different urotypes, some of which were dominated by potentially pathogenic species. Facultative pathogens however appear to be a common feature in patients without clinically manifested urinary tract infection.
trial registrationClinicalTrials.gov, NCT02845726 . Registered on 30 June 2016-retrospectively registered.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.