ArticleMicrobial biotechnology2019
Metabolic engineering of Bacillus subtilis for production of para-aminobenzoic acid - unexpected importance of carbon source is an advantage for space application.
Article in Microbial biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Microbial biopriming of germinated barley enhances phenolics and B-vitamins and improves neurobehavior in MSG-treated rats.NPJ science of food · 2026Article
- Enhanced extracellular respiration of engineered Bacillus subtilis via anodic electro-fermentation with pH optimisation.Biotechnology for biofuels and bioproducts · 2026Article
- Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida.Journal of biological engineering · 2025Article
- Isolation and characterization of aApplied and environmental microbiology · 2024Article
- Engineering Bacillus subtilis J46 for efficient utilization of galactose through adaptive laboratory evolution.AMB Express · 2024Article
- Microbial applications for sustainable space exploration beyond low Earth orbit.NPJ microgravity · 2023Review
- Microbial biomanufacturing for space-exploration-what to take and when to make.Nature communications · 2023Review
- Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions.Biotechnology for biofuels and bioproducts · 2023Article
- Aliivibrio fischeri L-Asparaginase production by engineered Bacillus subtilis: a potential new biopharmaceutical.Bioprocess and biosystems engineering · 2022Article
- Absence of increased genomic variants in the cyanobacterium Chroococcidiopsis exposed to Mars-like conditions outside the space station.Scientific reports · 2022Article
- Recent advances in the metabolic pathways and microbial production of coenzyme Q.World journal of microbiology & biotechnology · 2022Review
- Cultivation of the Dematiaceous FungusFrontiers in microbiology · 2022Article
- Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile.Frontiers in microbiology · 2021Article
- Advances on systems metabolic engineering ofSynthetic and systems biotechnology · 2020Article
- Metabolic engineering of Bacillus subtilis for production of para-aminobenzoic acid - unexpected importance of carbon source is an advantage for space application.Microbial biotechnology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-strength polymers, such as aramid fibres, are important materials in space technology. To obtain these materials in remote locations, such as Mars, biological production is of interest. The aromatic polymer precursor para-aminobenzoic acid (pABA) can be derived from the shikimate pathway through metabolic engineering of Bacillus subtilis, an organism suited for space synthetic biology. Our engineering strategy included repair of the defective indole-3-glycerol phosphate synthase (trpC), knockout of one chorismate mutase isozyme (aroH) and overexpression of the aminodeoxychorismate synthase (pabAB) and aminodeoxychorismate lyase (pabC) from the bacteria Corynebacterium callunae and Xenorhabdus bovienii respectively. Further, a fusion-protein enzyme (pabABC) was created for channelling of the carbon flux. Using adaptive evolution, mutants of the production strain, able to metabolize xylose, were created, to explore and compare pABA production capacity from different carbon sources. Rather than the efficiency of the substrate or performance of the biochemical pathway, the product toxicity, which was strongly dependent on the pH, appeared to be the overall limiting factor. The highest titre achieved in shake flasks was 3.22 g l
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