ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2019
CRISPR/Cas9-mediated genome editing reveals differences in the contribution of INDEHISCENT homologues to pod shatter resistance in Brassica napus L.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Goldilocks zone of lignin: Two extremes of valve lignification lead to silique indehiscence inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanisms of Silique Dehiscence in Rapeseed: A Review of Research Progress.Current issues in molecular biology · 2025Review
- Uncovering a stable QTLMolecular breeding : new strategies in plant improvement · 2025Article
- Cytological and genetic analyses of the seed number per silique locus BnSNSC09 in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- CRISPR mutant rapid identification inFrontiers in plant science · 2025Article
- Genome-wide identification of GH28 family and insight into its contributions to pod shattering resistance in Brassica napus L.BMC genomics · 2024Article
- The story of a decade: Genomics, functional genomics, and molecular breeding in Brassica napus.Plant communications · 2024Review
- The current scenario and future perspectives of transgenic oilseed mustard by CRISPR-Cas9.Molecular biology reports · 2023Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
- The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics.Plants (Basel, Switzerland) · 2022Review
- miR319-Regulated TCP3 Modulates Silique Development Associated with Seed Shattering in Brassicaceae.Cells · 2022Article
- Breeding history for shattering trait in sesame: classic to genomic approach.Molecular biology reports · 2022Review
- Identification and Characterization ofInternational journal of molecular sciences · 2022Article
- The application of CRISPR/Cas technologies toaBIOTECH · 2022Review
- CRISPR-Cas9-mediated editing of starch branching enzymes results in altered starch structure in Brassica napus.Plant physiology · 2022Article
- Site-Directed Mutagenesis of the Carotenoid Isomerase GeneFrontiers in plant science · 2022Article
- Functional Differentiation ofFrontiers in plant science · 2022Article
- USDA's revised biotechnology regulation's contribution to increasing agricultural sustainability and responding to climate change.Frontiers in plant science · 2022Review
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Authors and funding
7 authors.
Funding
Abstract
The INDEHISCENT (IND) and ALCATRAZ (ALC) gene homologues have been reported to be essential for dehiscence of fruits in Brassica species. But their functions for pod shatter resistance in Brassica napus, an important oil crops, are not well understood. Here, we assessed the functions of these two genes in rapeseed using CRISPR/Cas9 technology. The induced mutations were stably transmitted to successive generations, and a variety of homozygous mutants with loss-of-function alleles of the target genes were obtained for phenotyping. The results showed that the function of BnIND gene is essential for pod shatter and highly conserved in Brassica species, whereas the BnALC gene appears to have limited potential for rapeseed shatter resistance. The homoeologous copies of the BnIND gene have partially redundant roles in rapeseed pod shatter, with BnA03.IND exhibiting higher contributions than BnC03.IND. Analysis of data obtained from the gene expression and sequence variations of gene copies revealed that cis-regulatory divergences alter gene expression and underlie the functional differentiation of BnIND homologues. Collectively, our results generate valuable resources for rapeseed breeding programs, and more importantly provide a strategy to improve polyploid crops.
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