ArticleNature communications2019
Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Colorful 3D Reconstruction and an Extended Depth of Field for a Monocular Biological Microscope Using an Electrically Tunable Lens.Biomimetics (Basel, Switzerland) · 2024Article
- Cryo-EM elucidates the uroplakin complex structure within liquid-crystalline lipids in the porcine urothelial membrane.Communications biology · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.bioRxiv : the preprint server for biology · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.Research square · 2023Article
- The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.Nature communications · 2023Article
- Review
- CNG channel structure, function, and gating: a tale of conformational flexibility.Pflugers Archiv : European journal of physiology · 2021Review
- Macromolecular crystallography using microcrystal electron diffraction.Acta crystallographica. Section D, Structural biology · 2021Review
- Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Single Particle Analysis for High-Resolution 2D Electron Crystallography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Structural basis of Focal Adhesion Kinase activation on lipid membranes.The EMBO journal · 2020Article
- The Order-Disorder Continuum: Linking Predictions of Protein Structure and Disorder through Molecular Simulation.Scientific reports · 2020Review
- Advances in methods for atomic resolution macromolecular structure determination.F1000Research · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 Å were obtained from images of 2D crystals that do not diffract beyond 10 Å. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.