ArticleReproductive biology and endocrinology : RB&E2019
Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence.
Article in Reproductive biology and endocrinology : RB&E, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- Melatonin improves fertilization rate in assisted reproduction: Systematic review and meta-analysis.Clinics (Sao Paulo, Brazil) · 2024Pooled it
- Association between melatonin receptor gene polymorphisms and polycystic ovarian syndrome: a systematic review and meta-analysis.Bioscience reports · 2020Pooled it
- Methodological and reporting concerns regarding "Melatonin improves fertilization rate in assisted reproduction: systematic review and meta-analysis".Clinics (Sao Paulo, Brazil) · 2026Article
- Redox-endocrine triad in PCOS: can vitamin D, myo-inositol, and melatonin synergize as bioactive cocktails?Frontiers in endocrinology · 2026Review
- Investigating the effects of melatonin on structural and vascular changes in an experimentally induced ovarian hyperstimulation syndrome model : Effects of melatonin on experimentally induced ovarian hyperstimulation syndrome.Histochemistry and cell biology · 2025Article
- Exploring melatonin's multifaceted role in female reproductive health: From follicular development to lactation and its therapeutic potential in obstetric syndromes.Journal of advanced research · 2025Review
- Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries.Journal of personalized medicine · 2024Article
- Vitamin DIn vitro cellular & developmental biology. Animal · 2024Article
- Article
- The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Melatonin Alleviates Oxidative Stress Induced by HAntioxidants (Basel, Switzerland) · 2022Article
- Women with polycystic ovary syndrome (PCOS) have reduced melatonin concentrations in their follicles and have mild sleep disturbances.BMC women's health · 2022Article
- Melatonin and the Programming of Stem Cells.International journal of molecular sciences · 2022Review
- Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.Journal of cellular and molecular medicine · 2021Article
- Melatonin stimulates aromatase expression and estradiol production in human granulosa-lutein cells: relevance for high serum estradiol levels in patients with ovarian hyperstimulation syndrome.Experimental & molecular medicine · 2020Article
- Article
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7 authors at 1 institution in 1 country.
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Abstract
backgroundOvarian hyperstimulation syndrome (OHSS) is a common and severe complication for patients undergoing IVF/ICSI-ET. Melatonin widely participates in the regulation of female reproductive endocrine activity. However, whether melatonin participates in the progression of OHSS is largely unknown. This study aims to identify the predictive value of follicular fluid (FF) melatonin for OHSS establishment and the underlying mechanism.
methodsAll participants of this case-control study were enrolled at the Reproductive Medicine Center located in the First Affiliated Hospital of Zhengzhou University in China from January to October in 2017. Quantitative real-time PCR and western blot were used to examine the mRNA and protein levels. Primary granulosa cells were extracted and cultured for in vitro studies. Melatonin concentration was measured by ELISA. Logistic analysis and receiver-operating characteristic (ROC) curves were used to evaluate the predicting value of melatonin on OHSS occurrence.
main outcome measuresThe expression level of melatonin receptor 2 (MT2), P450 aromatase cytochrome (aromatase), vascular endothelial growth factor (VEGF), and inducible nitric oxide synthase (iNOS) mRNA in human primary granulosa cells. The concentration of melatonin in FF. The predicting value of melatonin on OHSS and the cut-off value of the prediction.
resultsFF melatonin concentrations were significantly higher in patients with OHSS compared to non-OHSS group (35.94 ± 10.18 ng/mL vs 23.93 ± 10.94 ng/mL, p<0.001). The expression of MT2 mRNA (p = 0.0459) and protein in granulosa cells was also significantly higher in the OHSS group. When using a cut-off level of 27.52 ng/ml, the sensitivity and specificity of FF melatonin to predict OHSS was 84.6 and 74.0%, respectively (p < 0.0001). We also found that melatonin could up-regulates aromatase mRNA, VEGF mRNA expression and down-regulates iNOS mRNA expression in the granulosa cells.
conclusionOHSS patients have higher melatonin in the FF as well as higher MT2 expression in the granulosa cells. The melatonin in FF might be used as an effective predictor for the occurrence of OHSS.
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