ArticleiScience2019
Imaging through the Whole Brain of Drosophila at λ/20 Super-resolution.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- LYNSU: automated 3D neuropil segmentation of fluorescent images forFrontiers in neuroinformatics · 2024Article
- Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adultFrontiers in neuroscience · 2022Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions.International journal of molecular sciences · 2021Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, many super-resolution technologies have been demonstrated, significantly affecting biological studies by observation of cellular structures down to nanometer precision. However, current super-resolution techniques mostly rely on wavefront engineering or wide-field imaging of signal blinking or fluctuation, and thus imaging depths are limited due to tissue scattering or aberration. Here we present a technique that is capable of imaging through an intact Drosophila brain with 20-nm lateral resolution at ∼200 μm depth. The spatial resolution is provided by molecular localization of a photoconvertible fluorescent protein Kaede, whose red form is found to exhibit blinking state. The deep-tissue observation is enabled by optical sectioning of spinning disk microscopy, as well as reduced scattering from optical clearing. Together these techniques are readily available for many biologists, providing three-dimensional resolution of densely entangled dendritic fibers in a complete Drosophila brain. The method paves the way toward whole-brain neural network studies and is applicable to other high-resolution bioimaging.
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Registered trials
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