ArticlePloS one2019
Effect of three different forms of handling on the variation of aggression-associated parameters in individually and group-housed male C57BL/6NCrl mice.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Laboratory conditions matter: social housing in male and female laboratory mice in a multicenter study approach.Lab animal · 2026Article
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- Lack of evidence for a consistent differential impact of tail and tunnel handling on markers of welfare in laboratory mice.Scientific reports · 2025Article
- Design and validation of novel flow cytometry panels to analyze a comprehensive range of peripheral immune cells in mice.Frontiers in immunology · 2024Article
- Molecular signature of excessive female aggression: study of stressed mice with genetic inactivation of neuronal serotonin synthesis.Journal of neural transmission (Vienna, Austria : 1996) · 2023Article
- Defensive and offensive behaviours in a Kleefstra syndrome mouse model.Animal cognition · 2023Article
- Individualized Housing Modifies the Immune-Endocrine System in CD1 Adult Male Mice.Animals : an open access journal from MDPI · 2023Article
- Experimenter familiarization is a crucial prerequisite for assessing behavioral outcomes and reduces stress in mice not only under chronic pain conditions.Scientific reports · 2023Article
- Bouts of rest and physical activity in C57BL/6J mice.PloS one · 2023Article
- Using refined methods to pick up mice: A survey benchmarking prevalence & beliefs about tunnel and cup handling.PloS one · 2023Article
- Article
- Impact of Refinements to Handling and Restraint Methods in Mice.Animals : an open access journal from MDPI · 2022Article
- Juvenile handling rescues autism-related effects of prenatal exposure to valproic acid.Scientific reports · 2022Article
- Selective Consumption of Fish Oil at End of the Day Increases the Physiological Fatty Acid Compositions of Eicosapentaenoic Acid and Docosahexaenoic Acid in Mice.Molecules (Basel, Switzerland) · 2022Article
- 'Invisible actors'-How poor methodology reporting compromises mouse models of oncology: A cross-sectional survey.PloS one · 2022Article
- Effects of non-aversive versus tail-lift handling on breeding productivity in a C57BL/6J mouse colony.PloS one · 2022Article
- Physical restraint mouse models to assess immune responses under stress with or without habituation.STAR protocols · 2021Article
- Aβ initiates brain hypometabolism, network dysfunction and behavioral abnormalities via NOX2-induced oxidative stress in mice.Communications biology · 2021Article
- Proteomics of Multiple Sclerosis: Inherent Issues in Defining the Pathoetiology and Identifying (Early) Biomarkers.International journal of molecular sciences · 2021Review
- Towards the Development of Long Circulating Phosphatidylserine (PS)- and Phosphatidylglycerol (PG)-Enriched Anti-Inflammatory Liposomes: Is PEGylation Effective?Pharmaceutics · 2021Article
Corrections and comments
- Expression of concern
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mice are social animals hence group-housing of mice is preferred over individual housing. However, aggression in group-housed male mice under laboratory housing conditions is a well-known problem leading to serious health issues, including injury or death. Therefore, group-housed mice are frequently separated for welfare reasons. In this study, we investigated the effect of 3 different handling methods (tail, forceps, tube) in 2 different housing conditions (single vs. group) on the variance of aggression-associated parameters in male C57BL/6NCrl mice over 8 weeks. Blood glucose concentration, body weight, body temperature, plus number and severity of bite wounds and barbering intensity in group-housed mice were recorded. An assessment of nest complexity was also performed weekly. Feces were collected in week 3 and 7 for analysis of corticosterone metabolites. We also monitored the level of aggression by recording the behavior of group-housed animals after weekly cage cleaning. An open field test followed by a social novel object test, a light/dark box test, a hotplate and a resident-intruder test were performed at the end of the 8-week handling period. Post-mortem, we assessed organ weights. We found that forceps-handled mice, independent of the housing condition, had significantly higher levels of stress-induced-hyperthermia and enhanced aggression after cage cleaning, and they performed worse in the nest complexity test. In addition, handling male mice by the tail seems to be most effective to reduce aggressiveness after transferring animals into new cages, thereby representing an appropriate refinement.
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