ArticleMethods in molecular biology (Clifton, N.J.)2019
In Ovo Electroporation of Plasmid DNA and Morpholinos into Specific Tissues During Early Embryogenesis.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- UnlockingNAR genomics and bioinformatics · 2026Article
- Core microRNAs regulate neural crest delamination and condensation in the developing trigeminal ganglion.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Modulation of mechanosensitive genes during embryonic aortic arch development.Developmental dynamics : an official publication of the American Association of Anatomists · 2025Article
- miR-203 secreted in extracellular vesicles mediates the communication between neural crest and placode cells required for trigeminal ganglia formation.PLoS biology · 2024Article
- Article
- The Axolotl's journey to the modern molecular era.Current topics in developmental biology · 2022Article
- Sox10-cre BAC transgenes reveal temporal restriction of mesenchymal cranial neural crest and identify glandular Sox10 expression.Developmental biology · 2021Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
In ovo electroporation enables transfection of non-viral plasmid DNA and/or morpholinos to fluorescently label and/or perturb gene function in cells of interest. However, targeted electroporation into specific subregions of the embryo can be challenging due to placement and size limitations of the electrodes. Here we describe the basic techniques for in ovo electroporation in the chick embryo and suggest parameters to electroporate cells within different target tissues that with some modifications may be applicable to a wide range of developmental stages and other embryo model organisms.
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Registered trials
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