ArticleScientific reports2019
A plasma membrane localized protein phosphatase in Toxoplasma gondii, PPM5C, regulates attachment to host cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- The Centrocone Protein SMC_N1 Is Important for the Proliferation ofAnimals : an open access journal from MDPI · 2026Article
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- Toxoplasma gondii PPM3H regulates the parasite virulence and modulates host immune and inflammatory responses in mice.Veterinary research · 2025Article
- Elucidating the Role ofBiomolecules · 2025Article
- Article
- Deletion of splicing factor Cdc5 in Toxoplasma disrupts transcriptome integrity, induces abortive bradyzoite formation, and prevents acute infection in mice.Nature communications · 2025Article
- Phosphatase UBLCP1 is required for the growth, virulence and mitochondrial integrity of Toxoplasma gondii.Parasites & vectors · 2025Article
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii.PLoS pathogens · 2024Article
- The essential kinase TgGSK regulates centrosome division and endodyogeny inbioRxiv : the preprint server for biology · 2024Article
- SPARK regulates AGC kinases central to theeLife · 2024Article
- SPARK regulates AGC kinases central to thebioRxiv : the preprint server for biology · 2024Article
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance inbioRxiv : the preprint server for biology · 2024Article
- The plant-like protein phosphatase PPKL regulates parasite replication and morphology in Toxoplasma gondii.Parasites & vectors · 2024Article
- The protein phosphatase PPKL is a key regulator of daughter parasite development inbioRxiv : the preprint server for biology · 2023Article
- Stable endocytic structures navigate the complex pellicle of apicomplexan parasites.Nature communications · 2023Article
- IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii.Journal of cell science · 2022Article
- Temporal and thermal profiling of theeLife · 2022Article
- The Tyrosine Phosphatase PRL Regulates Attachment of Toxoplasma gondii to Host Cells and Is Essential for Virulence.mSphere · 2022Article
- The Dually Localized EF-Hand Domain-Containing Protein TgEFP1 Regulates the Lytic Cycle ofCells · 2022Article
- Deciphering the Role of Protein Phosphatases in Apicomplexa: The Future of Innovative Therapeutics?Microorganisms · 2022Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The propagation of Toxoplasma gondii is accomplished by repeated lytic cycles of parasite attachment to a host cell, invasion, replication within a parasitophorous vacuole, and egress from the cell. This lytic cycle is delicately regulated by calcium-dependent reversible phosphorylation of the molecular machinery that drives invasion and egress. While much progress has been made elucidating the protein kinases and substrates central to parasite propagation, little is known about the relevant protein phosphatases. In this study, we focused on the five protein phosphatases that are predicted to be membrane-associated either integrally or peripherally. We have determined that of these only PPM5C, a PP2C family member, localizes to the plasma membrane of Toxoplasma. Disruption of PPM5C results in a slow propagation phenotype in tissue culture. Interestingly, parasites lacking PPM5C divide and undergo egress at a normal rate, but have a deficiency in attaching to host cells. Both membrane localization and phosphatase activity are required for PPM5C's role in attachment. Phosphoproteomic analysis show relatively few phosphorylation sites being affected by PPM5C deletion in extracellular parasites of which several are found on proteins involved in signaling cascades. This implies that PPM5C is part of a wider regulatory network important for attachment to host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.