ArticleThe Journal of biological chemistry2019
Nuclear transport of nicotinamide phosphoribosyltransferase is cell cycle-dependent in mammalian cells, and its inhibition slows cell growth.
Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- dCas9-metabolic enzyme fusions modulate global and locus-specific gene expression.bioRxiv : the preprint server for biology · 2026Article
- Cytoplasmic NAD/H synthesis via NRK1 regulates inflammatory capacity and promotes survival of CD4Nature communications · 2026Article
- Hallmarks of nuclear metabolism: implications for genome integrity, nuclear signaling, and therapeutic targeting.npj metabolic health and disease · 2026Review
- The role of NADnpj metabolic health and disease · 2025Review
- Subcellular NADNature metabolism · 2024Article
- Subcellular one carbon metabolism in cancer, aging and epigenetics.Frontiers in epigenetics and epigenomics · 2024Article
- Absence of mitochondrial SLC25A51 enhances PARP1-dependent DNA repair by increasing nuclear NAD+ levels.Nucleic acids research · 2023Article
- Targeting NADFrontiers in immunology · 2023Review
- Extracellular nicotinamide phosphoribosyltransferase: role in disease pathophysiology and as a biomarker.Frontiers in immunology · 2023Review
- Complex Positive Effects of SGLT-2 Inhibitor Empagliflozin in the Liver, Kidney and Adipose Tissue of Hereditary Hypertriglyceridemic Rats: Possible Contribution of Attenuation of Cell Senescence and Oxidative Stress.International journal of molecular sciences · 2021 · on this mapArticle
- Plasma level and expression of visfatin in the porcine hypothalamus during the estrous cycle and early pregnancy.Scientific reports · 2021Article
- Article
- Metabolic Fuel for Epigenetic: Nuclear Production Meets Local Consumption.Frontiers in genetics · 2021Review
- NAD metabolism in aging and cancer.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Nuclear metabolism and the regulation of the epigenome.Nature metabolism · 2020Review
- Article
- Location, Location, Location: Compartmentalization of NADTrends in biochemical sciences · 2020Review
- Two genomes, one cell: Mitochondrial-nuclear coordination via epigenetic pathways.Molecular metabolism · 2020Review
- A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NADThe Journal of biological chemistry · 2020Article
- NAMPT and NAPRT: Two Metabolic Enzymes With Key Roles in Inflammation.Frontiers in oncology · 2020Review
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Authors and funding
16 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinamide phosphoribosyltransferase (NAMPT) is located in both the nucleus and cytoplasm and has multiple biological functions including catalyzing the rate-limiting step in NAD synthesis. Moreover, up-regulated NAMPT expression has been observed in many cancers. However, the determinants and regulation of NAMPT's nuclear transport are not known. Here, we constructed a GFP-NAMPT fusion protein to study NAMPT's subcellular trafficking. We observed that in unsynchronized 3T3-L1 preadipocytes, 25% of cells had higher GFP-NAMPT fluorescence in the cytoplasm, and 62% had higher GFP-NAMPT fluorescence in the nucleus. In HepG2 hepatocytes, 6% of cells had higher GFP-NAMPT fluorescence in the cytoplasm, and 84% had higher GFP-NAMPT fluorescence in the nucleus. In both 3T3-L1 and HepG2 cells, GFP-NAMPT was excluded from the nucleus immediately after mitosis and migrated back into it as the cell cycle progressed. In HepG2 cells, endogenous, untagged NAMPT displayed similar changes with the cell cycle, and in nonmitotic cells, GFP-NAMPT accumulated in the nucleus. Similarly, genotoxic, oxidative, or dicarbonyl stress also caused nuclear NAMPT localization. These interventions also increased poly(ADP-ribosyl) polymerase and sirtuin activity, suggesting an increased cellular demand for NAD. We identified a nuclear localization signal in NAMPT and amino acid substitution in this sequence (
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