ArticleStem cells translational medicine2019
Generation of a Functioning and Self-Renewing Diaphragmatic Muscle Construct.
Article in Stem cells translational medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Extracellular Vesicles From Mesenchymal Stromal Cells Drive Muscle and Neuronal Regeneration Through TNFα Modulation.Journal of extracellular vesicles · 2026Article
- Comparison of different decellularization protocols for porcine centrum tendineum diaphragmatis and diaphragmatic muscle - a base for effective recellularization.Journal of biological engineering · 2026Article
- Tissue-engineered neuromuscular organoids.Communications biology · 2025Article
- Engineering Assembloids to Mimic Graft-Host Skeletal Muscle Interaction.Advanced healthcare materials · 2025Article
- Article
- Recent Advancements in Hydrogel Biomedical Research in Italy.Gels (Basel, Switzerland) · 2024Review
- 3D human induced pluripotent stem cell-derived bioengineered skeletal muscles for tissue, disease and therapy modeling.Nature protocols · 2023Review
- Article
- Bioprocessing by Decellularized Scaffold Biomaterials in Cultured Meat: A Review.Bioengineering (Basel, Switzerland) · 2022Review
- Effects of mitochondrial dysfunction on bone metabolism and related diseases: a scientometric study from 2003 to 2022.BMC musculoskeletal disorders · 2022Article
- Biomaterials for Regenerative Medicine in Italy: Brief State of the Art of the Principal Research Centers.International journal of molecular sciences · 2022Review
- Myoblast deactivation within engineered human skeletal muscle creates a transcriptionally heterogeneous population of quiescent satellite-like cells.Biomaterials · 2022Article
- Customized bioreactor enables the production of 3D diaphragmatic constructs influencing matrix remodeling and fibroblast overgrowth.NPJ Regenerative medicine · 2022Article
- Sources, Characteristics, and Therapeutic Applications of Mesenchymal Cells in Tissue Engineering.Tissue engineering and regenerative medicine · 2022Review
- Preclinical Development of Bioengineered Allografts Derived from Decellularized Human Diaphragm.Biomedicines · 2022Article
- 3D in vitro Models of Pathological Skeletal Muscle: Which Cells and Scaffolds to Elect?Frontiers in bioengineering and biotechnology · 2022Review
- Porcine Decellularized Diaphragm Hydrogel: A New Option for Skeletal Muscle Malformations.Biomedicines · 2021Article
- Extracellular matrix: an important regulator of cell functions and skeletal muscle development.Cell & bioscience · 2021Review
- Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.Stem cells translational medicine · 2020Article
- Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements.Materials (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
19 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surgical repair of large muscular defects requires the use of autologous graft transfer or prosthetic material. Naturally derived matrices are biocompatible materials obtained by tissue decellularization and are commonly used in clinical practice. Despite promising applications described in the literature, the use of acellular matrices to repair large defects has been only partially successful, highlighting the need for more efficient constructs. Scaffold recellularization by means of tissue engineering may improve not only the structure of the matrix, but also its ability to functionally interact with the host. The development of such a complex construct is challenging, due to the complexity of the native organ architecture and the difficulties in recreating the cellular niche with both proliferative and differentiating potential during growth or after damage. In this study, we tested a mouse decellularized diaphragmatic extracellular matrix (ECM) previously described by our group, for the generation of a cellular skeletal muscle construct with functional features. The decellularized matrix was stored using different conditions to mimic the off-the-shelf clinical need. Pediatric human muscle precursors were seeded into the decellularized scaffold, demonstrating proliferation and differentiation capability, giving rise to a functioning three-dimensional skeletal muscle structure. Furthermore, we exposed the engineered construct to cardiotoxin injury and demonstrated its ability to activate a regenerative response in vitro promoting cell self-renewal and a positive ECM remodeling. Functional reconstruction of an engineered skeletal muscle with maintenance of a stem cell pool makes this a promising tool toward future clinical applications in diaphragmatic regeneration. Stem Cells Translational Medicine 2019;8:858&869.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.