ArticlePlant biotechnology journal2019
Efficient genetic transformation and CRISPR/Cas9-mediated genome editing in Lemna aequinoctialis.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Duckweed Proteins in Neuro-Nutrition: Mechanistic Insights and Translational Relevance for Military and Extreme Environments.Plant foods for human nutrition (Dordrecht, Netherlands) · 2026Review
- Duckweeds: from fundamental biology to a sustainable plant chassis for biotechnology.Advanced biotechnology · 2026Review
- Targeted multiplex gene knockouts in Lemna minor using CRISPR/Cas9.Transgenic research · 2026Article
- Strain, procedures, and tools for reproducible genetic transformation and genome editing of the emerging plant model Spirodela polyrhiza.The New phytologist · 2026Article
- Exploring Duckweed Diversity at the Dawn of Its Cultivation Era: The Invaluable Legacy of the Landolt Collection.Plants (Basel, Switzerland) · 2026Article
- Article
- Duckweeds: Model organisms for research on plant sterols and steroids.Plant biology (Stuttgart, Germany) · 2026Review
- Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies forbioRxiv : the preprint server for biology · 2025Article
- 7th International Conference on Duckweed Research and Applications: Depicting an Era of Advancing Research Translation Toward Practical Applications.Plants (Basel, Switzerland) · 2025Article
- Developments in Toxicity Testing with Duckweeds.Journal of xenobiotics · 2025Review
- Utilization of Microalgae and Duckweed as Sustainable Protein Sources for Food and Feed: Nutritional Potential and Functional Applications.Journal of agricultural and food chemistry · 2025Review
- Enzymatic Characterization ofPlants (Basel, Switzerland) · 2024Article
- Duckweed: Beyond an Efficient Plant Model System.Biomolecules · 2024Review
- An Ionic Liquid-Based Biorefinery Approach for Duckweed Utilization.ACS sustainable resource management · 2024Article
- CRISPR/Cas9 ribonucleoprotein mediated DNA-free genome editing in larch.Forestry research · 2024Article
- Mechanisms of metabolic adaptation in the duckweed Lemna gibba: an integrated metabolic, transcriptomic and flux analysis.BMC plant biology · 2023Article
- Cryopreservation of Duckweed Genetic Diversity as Model for Long-Term Preservation of Aquatic Flowering Plants.Plants (Basel, Switzerland) · 2023Article
- Characterisation of a Spontaneous Mutant ofPlants (Basel, Switzerland) · 2023Article
- Sixth International Conference on Duckweed Research and Applications Presents Lemnaceae as a Model Plant System in the Genomics and Postgenomics Era.Plants (Basel, Switzerland) · 2023Article
- Engineering triacylglycerol accumulation in duckweed (Lemna japonica).Plant biotechnology journal · 2023Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fast growth, ease of metabolic labelling and potential for feedstock and biofuels production make duckweeds not only an attractive model system for understanding plant biology, but also a potential future crop. However, current duckweed research is constrained by the lack of efficient genetic manipulation tools. Here, we report a case study on genome editing in a duckweed species, Lemna aequinoctialis, using a fast and efficient transformation and CRISPR/Cas9 tool. By optimizing currently available transformation protocols, we reduced the duration time of Agrobacterium-mediated transformation to 5-6 weeks with a success rate of over 94%. Based on the optimized transformation protocol, we generated 15 (14.3% success rate) biallelic LaPDS mutants that showed albino phenotype using a CRISPR/Cas9 system. Investigations on CRISPR/Cas9-mediated mutation spectrum among mutated L. aequinoctialis showed that most of mutations were short insertions and deletions. This study presents the first example of CRISPR/Cas9-mediated genome editing in duckweeds, which will open new research avenues in using duckweeds for both basic and applied research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.