ArticleScientific reports2019
A Comprehensive Biophysical Analysis of the Effect of DNA Binding Drugs on Protamine-induced DNA Condensation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Protamine Drives Liquid and Solid Condensation of DNA and Glycosaminoglycans.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Polyethylene Glycolylation of the Purified Basic Protein (Protamine) of Squid (International journal of molecular sciences · 2025Article
- A Minor Groove Binder with Significant Cytotoxicity on Human Lung Cancer Cells: The Potential of Hesperetin Functionalised Silver Nanoparticles.Journal of fluorescence · 2024Article
- New Insights into the Role of Ligand-Binding Modes in GC-DNA Condensation through Thermodynamic and Spectroscopic Studies.ACS omega · 2023Article
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- Delineation of the Residues of Bacillus anthracis Zinc Uptake Regulator Protein Directly Involved in Its Interaction with Cognate DNA.Biological trace element research · 2021Article
- Biopolymer-based Carriers for DNA Vaccine Design.Angewandte Chemie (International ed. in English) · 2021Review
- DNA Condensation Triggered by the Synergistic Self-Assembly of Tetraphenylethylene-Viologen Aggregates and CT-DNA.Frontiers in chemistry · 2021Article
- The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents.Research in pharmaceutical sciences · 2020Article
- Nucleotide-Bearing Benzylidene-Tetrahydroxanthylium Near-IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Can the Isothermal Calorimetric Curve Shapes Suggest the Structural Changes in Micellar Aggregates?International journal of molecular sciences · 2020Article
- Ultrasound-assisted multicomponent synthesis of 4H-pyrans in water and DNA binding studies.Scientific reports · 2020Article
- Estimation of a stronger heparin binding locus in fibronectin domain IIIRSC advances · 2020Article
- A Low-Cost Time-Correlated Single Photon Counting Portable DNA Analyzer.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA condensation is a ubiquitous phenomenon in biology, yet the physical basis for it has remained elusive. Here, we have explored the mechanism of DNA condensation through the protamine-DNA interaction, and by examining on it the influence of DNA binding drugs. We observed that the DNA condensation is accompanied by B to Ψ-DNA transition as a result of DNA base pair distortions due to protamine binding, bringing about the formation of toroidal structure through coil-globule transition. The binding energetics suggested that electrostatic energy, bending energy and hydration energy must play crucial roles in DNA condensation. EtBr intercalation interferes with the protamine-DNA interaction, challenging the distortion of the DNA helix and separation of DNA base pairs by protamine. Thus, EtBr, by competing directly with protamine, resists the phenomenon of DNA condensation. On the contrary, netropsin impedes the DNA condensation by an allosteric mechanism, by resisting the probable DNA major groove bending by protamine. In summary, we demonstrate that drugs with distinct binding modes use different mechanism to interfere with DNA condensation.
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