ArticleNature communications2019
Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Three-color high-definition whole-brain imaging: simultaneous analysis of multiple anatomical structures with colocalized cytoarchitectural information.Biomedical optics express · 2026Article
- Multicolor Cell Lineage Tracing Using MAGIC Markers Strategies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Population imaging of internal state circuits relevant to psychiatric disease: a review.Neurophotonics · 2025Review
- Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation.Npj imaging · 2024Article
- Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.Biomedical optics express · 2024Article
- Three-Dimensional Imaging of Bioinspired Lipidic Mesophases Using Multicolored Light-Emitting Carbon Nanodots.The journal of physical chemistry letters · 2024Article
- Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window.bioRxiv : the preprint server for biology · 2024Article
- Size and Illumination Matters: Local Magnetic Actuation and Fluorescence Imaging for Microrobotics.Journal of the Indian Institute of Science · 2024Review
- NBS-SNI, an extension of the network-based statistic: Abnormal functional connections between important structural actors.Network neuroscience (Cambridge, Mass.) · 2024Article
- Visualization and Characterization of the Brain Regional Heterogeneity of Astrocyte-Astrocyte Structural Interactions by Using Improved Iontophoresis with Dual-Fluorescent Dyes.Brain sciences · 2023Article
- A deep learning-based stripe self-correction method for stitched microscopic images.Nature communications · 2023Article
- Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus.Nature communications · 2023Article
- Protein nanopore reveals the renin-angiotensin system crosstalk with single-amino-acid resolution.Nature chemistry · 2023Article
- Modern Microscopic Approaches to Astrocytes.International journal of molecular sciences · 2023Review
- Distinctive biophysical features of human cell-types: insights from studies of neurosurgically resected brain tissue.Frontiers in synaptic neuroscience · 2023Review
- Two-photon microscopy for microrobotics: Visualization of micro-agents below fixed tissue.PloS one · 2023Article
- Interpretable Classification of Tauopathies with a Convolutional Neural Network Pipeline Using Transfer Learning and Validation against Post-Mortem Clinical Cases of Alzheimer's Disease and Progressive Supranuclear Palsy.Current issues in molecular biology · 2022Article
- Article
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
- Multiple forms of working memory emerge from synapse-astrocyte interactions in a neuron-glia network model.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.