Evidence map›Paper›PMID 30971308›Full record

ArticleBMC research notes2019

Bacillus subtilis RarA forms damage-inducible foci that scan the entire cell.

Rogelio Hernández-Tamayo, Peter L Graumann

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. "ThebioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
  5. Frontiers in microbiology · 2020
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Rogelio Hernández-TamayoSYNMIKRO, LOEWE Center for Synthetic Microbiology, Marburg, Germany.
Peter L GraumannSYNMIKRO, LOEWE Center for Synthetic Microbiology, Marburg, Germany. graumanp@uni-marburg.de.ORCID http://orcid.org/0000-0002-8033-5171
Philipps University of Marburg · DE

Funding

Deutsche Forschungsgemeinschaft TRR174
6 · The paper itself

Abstract

objectivesLittle is known about the activity and dynamics of ATPase RarA in B. subtilis, proposed to act at stalled DNA replication forks due to DNA damage. We performed fluorescence microscopy time lapse experiments with a functional RarA-mVenus fusion to visualize the dynamics of RarA during conditions that generate DNA damage. DATA DESCRIPTION: In exponentially growing cells, we observed that 15% of the cells contained single RarA-mV (mVenus fluorescent fusion) foci moving throughout the entire cell between 3 min intervals. This percentage remained constant at different time points, indicating that focus formation during unperturbed growth is maintained at about a constant rate. When cells were exposed to stress conditions, the population of cells containing RarA-mV foci tripled after 60 min. Cells exposed to two DNA-damaging drugs, to 5 mM MMS or to 0.5 mM H

Indexed as

DNA RepairGene Expression Regulation, BacterialAdenosine TriphosphatasesBacillus subtilisBacterial ProteinsCell CycleDNA, BacterialDNA-Binding ProteinsDNA DamageGenes, ReporterHydrogen PeroxideLuminescent ProteinsMicroscopy, FluorescenceProtein TransportRecombinant Fusion ProteinsStress, PhysiologicalAdenosine TriphosphatasesBacterial ProteinsDNA, BacterialDNA-Binding ProteinsHydrogen PeroxideLuminescent Proteinsmethyl methanethiosulfinateRecombinant Fusion ProteinsSulfinic Acidsyellow fluorescent protein, BacteriaBacillus subtilisDNA repairRarAStress responseTime lapse fluorescence microscopy

Identifiers

PMID30971308
PMCPMC6458690
OpenAlexW2942832294

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.