Evidence map›Paper›PMID 30971289›Full record

ArticleVeterinary research2019

Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates.

Annelies Maria Declercq, Wenlong Cai, Eber Naranjo, Wilawan Thongda, Venessa Eeckhaut, Eva Bauwens, Covadonga Arias, Leonardo De La Fuente, Benjamin H Beck, Miles D Lange and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Pleiotropic effects of signal peptide peptidase A (Frontiers in veterinary science · 2026
    Article
  3. Cortisol Promotes Surface Translocation ofPathogens (Basel, Switzerland) · 2022
    Article
  4. Article
  5. Surface Properties ofMicroorganisms · 2021
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Annelies Maria DeclercqDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, 9000, Belgium. andclerc.declercq@ugent.be.ORCID http://orcid.org/0000-0002-6041-9597
Wenlong CaiSchool of Fisheries, Aquaculture, and Aquatic Sciences, Aquatic Microbiology Laboratory, Auburn University, Auburn, AL, 36849, USA.
Eber NaranjoDepartment of Entomology and Plant Pathology, Auburn University, Auburn, AL, 36849, USA.
Wilawan ThongdaSchool of Fisheries, Aquaculture, and Aquatic Sciences, Aquatic Genetics and Genomics, Auburn University, Auburn, AL, 36849, USA.
Venessa EeckhautDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, 9000, Belgium.
Eva BauwensDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, 9000, Belgium.
Covadonga AriasSchool of Fisheries, Aquaculture, and Aquatic Sciences, Aquatic Microbiology Laboratory, Auburn University, Auburn, AL, 36849, USA.
Leonardo De La FuenteDepartment of Entomology and Plant Pathology, Auburn University, Auburn, AL, 36849, USA.
Benjamin H BeckUnited States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, Auburn, AL, 36849, USA.
Miles D LangeUnited States Department of Agriculture, Agricultural Research Service, Harry K. Dupree Stuttgart National Aquaculture Research Center, Stuttgart, AR, 72160, USA.
Eric PeatmanSchool of Fisheries, Aquaculture, and Aquatic Sciences, Aquatic Genetics and Genomics, Auburn University, Auburn, AL, 36849, USA.
Freddy HaesebrouckDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, 9000, Belgium.
Johan AertsStress Physiology Research Group, Faculty of Pharmaceutical Sciences, Ghent University, Ostend, 8400, Belgium.
Annemie DecostereDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, 9000, Belgium.
Auburn University · USGhent University · BEAgricultural Research Service · US

Funding

Bijzonder Onderzoeksfonds BOF.PDO.2015.0020.01Fonds Wetenschappelijk Onderzoek V423316N
6 · The paper itself

Abstract

The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.

Indexed as

Gene ExpressionAnimalsBacterial AdhesionBiofilmsCarpsDose-Response Relationship, DrugFlavobacteriumGenes, BacterialHydrocortisoneLab-On-A-Chip DevicesPlanktonVirulenceHydrocortisone

Identifiers

PMID30971289
PMCPMC6458827
OpenAlexW2945156658

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.