ArticlePolymers2017
Primary Hepatocytes Cultured on a Fiber-Embedded PDMS Chip to Study Drug Metabolism.
Article in Polymers, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Proposal for a non-adhesive single-cell culture technology for primary hepatocytes.Cytotechnology · 2025Article
- Current strategies with implementation of three-dimensional cell culture: the challenge of quantification.Interface focus · 2022Review
- CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip.Materials (Basel, Switzerland) · 2021Review
- The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review.Micromachines · 2020Review
- A microfluidic 3D hepatocyte chip for hepatotoxicity testing of nanoparticles.Nanomedicine (London, England) · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro drug screening using reliable and predictable liver models remains a challenge. The identification of an ideal biological substrate is essential to maintain hepatocyte functions during in vitro culture. Here, we developed a fiber-embedded polydimethylsiloxane (PDMS) chip to culture hepatocytes. Hepatocyte spheroids formed in this device were subjected to different flow rates, of which a flow rate of 50 μL/min provided the optimal microenvironment for spheroid formation, maintained significantly higher rates of albumin and urea synthesis, yielded higher CYP3A1 (cytochrome P450 3A1) and CYP2C11 (cytochrome P450 2C11) enzyme activities for metabolism, and demonstrated higher expression levels of liver-specific genes. In vitro metabolism tests on tolbutamide and testosterone by hepatocytes indicated predicted clearance rates of 1.98 ± 0.43 and 40.80 ± 10.13 mL/min/kg, respectively, which showed a good in vitro⁻in vivo correspondence. These results indicate that this system provides a strategy for the construction of functional engineered liver tissue that can be used to study drug metabolism.
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Registered trials
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