ArticleJournal of neural engineering2019
PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices.
Article in Journal of neural engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Mutation E300 recommended by protein language models gives ChrimsonR amplified photocurrent response.bioRxiv : the preprint server for biology · 2026Article
- High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons.Nature protocols · 2026Review
- The evolution of patch-clamp electrophysiology: Robotic, multiplex, and dynamic.Molecular pharmacology · 2025Review
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties.American journal of physiology. Cell physiology · 2023Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Electrophysiological and morphological characterization of single neurons in intact human brain organoids.Journal of neuroscience methods · 2023Article
- Article
- High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.Cell reports methods · 2023Article
- From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research.Frontiers in pharmacology · 2023Review
- Biophysical characterization of light-gated ion channels using planar automated patch clamp.Frontiers in molecular neuroscience · 2022Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
- Automated Intracellular Pharmacological Electrophysiology for Ligand-Gated Ionotropic Receptor and Pharmacology Screening.Molecular pharmacology · 2021Article
- Deep learning-based real-time detection of neurons in brain slices for in vitro physiology.Scientific reports · 2021Article
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Compensation of physiological motion enables high-yield whole-cell recording in vivo.Journal of neuroscience methods · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
objectiveIntracellular patch-clamp electrophysiology, one of the most ubiquitous, high-fidelity techniques in biophysics, remains laborious and low-throughput. While previous efforts have succeeded at automating some steps of the technique, here we demonstrate a robotic 'PatcherBot' system that can perform many patch-clamp recordings sequentially, fully unattended. APPROACH: Comprehensive automation is accomplished by outfitting the robot with machine vision, and cleaning pipettes instead of manually exchanging them. MAIN
resultsthe PatcherBot can obtain data at a rate of 16 cells per hour and work with no human intervention for up to 3 h. We demonstrate the broad applicability and scalability of this system by performing hundreds of recordings in tissue culture cells and mouse brain slices with no human supervision. Using the PatcherBot, we also discovered that pipette cleaning can be improved by a factor of three. SIGNIFICANCE: The system is potentially transformative for applications that depend on many high-quality measurements of single cells, such as drug screening, protein functional characterization, and multimodal cell type investigations.
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